{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T11:40:47Z","timestamp":1772624447531,"version":"3.50.1"},"reference-count":75,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T00:00:00Z","timestamp":1547078400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004564","name":"Ministarstvo Prosvete, Nauke i Tehnolo\u0161kog Razvoja","doi-asserted-by":"publisher","award":["173033"],"award-info":[{"award-number":["173033"]}],"id":[{"id":"10.13039\/501100004564","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Endocrinology"],"published-print":{"date-parts":[[2019,1,10]]},"abstract":"<jats:p>This study aimed at examining the early effects of hyperbaric oxygen therapy (HBOT) on inducible nitric oxide synthase (iNOS) activity\/expression in lymphocytes of type 1 diabetes mellitus (T1DM) patients. A group of 19 patients (mean age: 63\u2009\u00b1\u20092.1) with T1DM and with the peripheral arterial disease were included in this study. Patients were exposed to 10 sessions of HBOT in the duration of 1\u2009h to 100% oxygen inhalation at 2.4\u2009ATA. Blood samples were collected for the plasma C-reactive protein (CRP), plasma free fatty acid (FFA), serum nitrite\/nitrate, and serum arginase activity measurements. Expression of iNOS and phosphorylation of p65 subunit of nuclear factor-<jats:italic>\u03ba<\/jats:italic>B (NF<jats:italic>\u03ba<\/jats:italic>B-p65), extracellular-regulated kinases 1\/2 (ERK1\/2), and protein kinase B (Akt) were examined in lymphocyte lysates by Western blot. After exposure to HBOT, plasma CRP and FFA were significantly decreased (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.001<\/mml:mn><\/mml:math>). Protein expression of iNOS and serum nitrite\/nitrate levels were decreased (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:math>), while serum arginase activity was increased (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>) versus before exposure to HBOT. Increased phosphorylation of NF<jats:italic>\u03ba<\/jats:italic>B-p65 at Ser<jats:sup>536<\/jats:sup> (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>) and decreased level of NF<jats:italic>\u03ba<\/jats:italic>B-p65 protein (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.001<\/mml:mn><\/mml:math>) in lymphocytes of T1DM patients were observed after HBOT. Decreased phosphorylation of ERK1\/2 (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>) and Akt (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\"><mml:mi>p<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>) was detected after HBOT. Our results indicate that exposure to HBO decreased iNOS activity\/expression via decreasing phosphorylation of ERK1\/2 and Akt followed by decreased activity of NF<jats:italic>\u03ba<\/jats:italic>B.<\/jats:p>","DOI":"10.1155\/2019\/2328505","type":"journal-article","created":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T18:31:01Z","timestamp":1547145061000},"page":"1-12","source":"Crossref","is-referenced-by-count":16,"title":["Early Effects of Hyperbaric Oxygen on Inducible Nitric Oxide Synthase Activity\/Expression in Lymphocytes of Type 1 Diabetes Patients: A Prospective Pilot Study"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2742-7912","authenticated-orcid":true,"given":"Ivana","family":"Resanovic","sequence":"first","affiliation":[{"name":"Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6371-6610","authenticated-orcid":true,"given":"Zoran","family":"Gluvic","sequence":"additional","affiliation":[{"name":"Clinic for Internal Medicine, Zemun Clinical Hospital, School of Medicine, University of Belgrade, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bozidarka","family":"Zaric","sequence":"additional","affiliation":[{"name":"Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2273-5310","authenticated-orcid":true,"given":"Emina","family":"Sudar-Milovanovic","sequence":"additional","affiliation":[{"name":"Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8809-8951","authenticated-orcid":true,"given":"Aleksandra","family":"Jovanovic","sequence":"additional","affiliation":[{"name":"Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davorka","family":"Milacic","sequence":"additional","affiliation":[{"name":"Department of Hyperbaric Medicine, Zemun Clinical Hospital, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radmilo","family":"Isakovic","sequence":"additional","affiliation":[{"name":"Department of Hyperbaric Medicine, Zemun Clinical Hospital, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0012-2636","authenticated-orcid":true,"given":"Esma R.","family":"Isenovic","sequence":"additional","affiliation":[{"name":"Institute of Nuclear Sciences Vinca, University of Belgrade, Laboratory of Radiobiology and Molecular Genetics, Belgrade, Serbia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-1326.2006.00655.x"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.2522\/ptj.20080008"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(95)00522-6"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2016.09.009"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1038\/ncpendmet0366"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.5.1340"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.2337\/db09-0432"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2010.05.003"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-06-0291"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1662308"},{"key":"11","volume-title":"Physiological, and biochemical aspects of hyperbaric oxygenation","year":"2009"},{"key":"12","first-page":"14","volume-title":"Physical and pathophysiological bases of hyperbaric oxygen therapy","year":"2006"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1136\/gut.43.4.512"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/147043"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2016.02.007"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/0009-8981(64)90004-X"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)90027-2"},{"key":"18","first-page":"77","volume":"97","year":"1968","journal-title":"Scandinavian Journal of Clinical and Laboratory Investigation. 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