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Porcine rickets with weak limbs and hooves bring huge economic losses to the pig industry. However, research on the development of pig cartilage is lacking. This study investigated the key genes and molecular mechanisms involved in cartilage development via an RNA-seq technique. Samples of proximal tibia cartilage were collected from three normal piglets with 1 day, 14 days, and 28 days of age, respectively, and then these samples were divided into two comparison groups (1-day vs. 14-day group, 14-day vs. 28-day group). Through the transcriptome analysis, 108 differentially expressed genes (DEGs), such as <jats:italic>FORL2<\/jats:italic>, were obtained from 1-day vs. 14-day comparison group, and 3602 DEGs were obtained from 14-day vs. 28-day comparison group, including <jats:italic>SOX9<\/jats:italic>, <jats:italic>BMP6<\/jats:italic>, and <jats:italic>MMP13<\/jats:italic>. The gene ontology (GO) functional and KEGG pathway enrichment revealed that many functions of DEGs were related to bone development. The pathways of DEGs from Day 1 vs. Day 14 were mainly enriched in mineral absorption, but the DEGs of Day 14 vs. Day 28 were enriched in osteoclast differentiation. Then, the expression patterns of six candidate genes were verified via qPCR. In conclusion, candidate genes affecting cartilage development in Yorkshire pigs were obtained by transcriptome analysis, and the clues showed that Day 14 to Day 28 is a more active and extensive period in cartilage developments, which played a key role in revealing the molecular mechanism of pig cartilage development basis, also compensating for vacancies in cartilage research.<\/jats:p>","DOI":"10.1155\/2019\/2365416","type":"journal-article","created":{"date-parts":[[2019,11,5]],"date-time":"2019-11-05T18:30:56Z","timestamp":1572978656000},"page":"1-12","source":"Crossref","is-referenced-by-count":1,"title":["Identification of mRNAs Related to Tibial Cartilage Development of Yorkshire Piglets"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4618-0217","authenticated-orcid":true,"given":"Shuaifei","family":"Feng","sequence":"first","affiliation":[{"name":"Key Laboratory of Animal Genetics, Breeding and Reproduction of Ministry of Education, 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