{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T02:54:23Z","timestamp":1771556063113,"version":"3.50.1"},"reference-count":40,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,12,22]],"date-time":"2019-12-22T00:00:00Z","timestamp":1576972800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002338","name":"Ministry of Education of the People's Republic of China","doi-asserted-by":"publisher","award":["2019kfkt05"],"award-info":[{"award-number":["2019kfkt05"]}],"id":[{"id":"10.13039\/501100002338","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002338","name":"Ministry of Education of the People's Republic of China","doi-asserted-by":"publisher","award":["A2019471"],"award-info":[{"award-number":["A2019471"]}],"id":[{"id":"10.13039\/501100002338","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002338","name":"Ministry of Education of the People's Republic of China","doi-asserted-by":"publisher","award":["2016KZDXM033"],"award-info":[{"award-number":["2016KZDXM033"]}],"id":[{"id":"10.13039\/501100002338","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002338","name":"Ministry of Education of the People's Republic of China","doi-asserted-by":"publisher","award":["81771696"],"award-info":[{"award-number":["81771696"]}],"id":[{"id":"10.13039\/501100002338","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Medical Research Fund of Guangdong Province","award":["2019kfkt05"],"award-info":[{"award-number":["2019kfkt05"]}]},{"name":"Medical Research Fund of Guangdong Province","award":["A2019471"],"award-info":[{"award-number":["A2019471"]}]},{"name":"Medical Research Fund of Guangdong Province","award":["2016KZDXM033"],"award-info":[{"award-number":["2016KZDXM033"]}]},{"name":"Medical Research Fund of Guangdong Province","award":["81771696"],"award-info":[{"award-number":["81771696"]}]},{"DOI":"10.13039\/501100010226","name":"Guangdong Provincial Education Department","doi-asserted-by":"crossref","award":["2019kfkt05"],"award-info":[{"award-number":["2019kfkt05"]}],"id":[{"id":"10.13039\/501100010226","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010226","name":"Guangdong Provincial Education Department","doi-asserted-by":"crossref","award":["A2019471"],"award-info":[{"award-number":["A2019471"]}],"id":[{"id":"10.13039\/501100010226","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010226","name":"Guangdong Provincial Education Department","doi-asserted-by":"crossref","award":["2016KZDXM033"],"award-info":[{"award-number":["2016KZDXM033"]}],"id":[{"id":"10.13039\/501100010226","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010226","name":"Guangdong Provincial Education Department","doi-asserted-by":"crossref","award":["81771696"],"award-info":[{"award-number":["81771696"]}],"id":[{"id":"10.13039\/501100010226","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2019kfkt05"],"award-info":[{"award-number":["2019kfkt05"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["A2019471"],"award-info":[{"award-number":["A2019471"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2016KZDXM033"],"award-info":[{"award-number":["2016KZDXM033"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771696"],"award-info":[{"award-number":["81771696"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2019,12,22]]},"abstract":"<jats:p>Toll-like receptors (TLRs) play an important role in regulating immune responses during pathogen infection. However, roles of TLRs on T cells reside in the mesenteric lymph node (MLN) were not be fully elucidated in the course of <jats:italic>S. japonicum<\/jats:italic> infection. In this study, T lymphocytes from the mesenteric lymph node (MLN) of <jats:italic>S. japonicum<\/jats:italic>-infected mice were isolated and the expression and roles of TLR2, TLR3, TLR4, and TLR7 on both CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells were compared. We found that the expression of TLR7 was increased in the MLN cells of <jats:italic>S. japonicum<\/jats:italic>-infected mice, particularly in CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). R848, a TLR7 agonist, could enhance the production of IFN-<jats:italic>\u03b3<\/jats:italic> from MLN T cells of infected mice (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>), especially in CD8<jats:sup>+<\/jats:sup> T cells (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:math>). In TLR7 gene knockedout (KO) mice, the <jats:italic>S. japonicum<\/jats:italic> infection caused a significant decrease (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>) of the expression of CD25 and CD69, as well as the production of IFN-<jats:italic>\u03b3<\/jats:italic> and IL-4 inducted by PMA plus ionomycin on both CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T cells. Furthermore, the decreased level of IFN-<jats:italic>\u03b3<\/jats:italic> and IL-4 in the supernatants of SEA- or SWA-stimulated mesenteric lymphocytes was detected (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>). Our results indicated that <jats:italic>S. japonicum<\/jats:italic> infection could induce the TLR7 expression on T cells in the MLN of C57BL\/6 mice, and TLR7 mediates T cell response in the early phase of infection.<\/jats:p>","DOI":"10.1155\/2019\/2691808","type":"journal-article","created":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T07:58:34Z","timestamp":1582185514000},"page":"1-14","source":"Crossref","is-referenced-by-count":6,"title":["TLR7 Modulated T Cell Response in the Mesenteric Lymph Node of <i>Schistosoma japonicum<\/i>-Infected C57BL\/6 Mice"],"prefix":"10.1155","volume":"2019","author":[{"given":"Jiale","family":"Qu","sequence":"first","affiliation":[{"name":"The Second Affiliated Hospital, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510260, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuxue","family":"Yu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510260, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenxi","family":"Jin","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510260, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanfa","family":"Feng","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510260, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shihao","family":"Xie","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510260, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyan","family":"Xie","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510260, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan","family":"Yang","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510260, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanwei","family":"Qi","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510260, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huaina","family":"Qiu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510260, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongyuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Guangdong Pharmaceutical University, Guangzhou 510006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6012-8389","authenticated-orcid":true,"given":"Jianbing","family":"Mu","sequence":"additional","affiliation":[{"name":"Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0704-1510","authenticated-orcid":true,"given":"Yi","family":"Zhou","sequence":"additional","affiliation":[{"name":"College of Pharmacy, Guangzhou Medical University, 510182 Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6135-1256","authenticated-orcid":true,"given":"Jun","family":"Huang","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangdong Provincial Key Laboratory of Allergy & Clinical Immunology, The State Key Laboratory of Respiratory Disease, Guangzhou Medical University, Guangzhou 510260, China"},{"name":"Key Laboratory of Tropical Diseases Control (Sun Yat-sen University), Ministry of Education, Guangzhou, Guangdong 510080, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-012-3104-6"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.icb.7100014"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2013.09.008"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12105"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3350-2"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1038\/nri843"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.03042"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200425027"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1093\/ecco-jcc\/jjy136"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2019.01.005"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-013-3732-5"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-014-4004-8"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.1RI0418-152RR"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-017-5678-5"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-02422-8"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12623"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1038\/nri3446"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206941200"},{"issue":"3","key":"19","first-page":"172","volume":"32","year":"2014","journal-title":"Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2009.03143.x"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxf069"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1126\/stke.4022007pe48"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.4110\/in.2009.9.4.127"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.3.1551"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2018.01.014"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1242\/dmm.027409"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2018.195628"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2019.03.113"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2010.06.001"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2018.01.077"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2018.07.014"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.11.003"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.14234"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001269"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2019.07.45"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900052"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1038\/cti.2016.22"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1038\/90609"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2011.02.024"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxv017"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2019\/2691808.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2019\/2691808.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2019\/2691808.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,2,20]],"date-time":"2020-02-20T07:59:12Z","timestamp":1582185552000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jir\/2019\/2691808\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,22]]},"references-count":40,"alternative-id":["2691808","2691808"],"URL":"https:\/\/doi.org\/10.1155\/2019\/2691808","relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,22]]}}}