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While there are PCR assays that can identify individual or groups of<jats:italic>Bartonella<\/jats:italic>, there is no reliable molecular method to simultaneously detect all species while maintaining genus specificity and sensitivity. By comparing highly conserved<jats:italic>16S rRNA<\/jats:italic>sequences of the better-recognized<jats:italic>Bartonella<\/jats:italic>spp. on GenBank, we selected primers and probes for a genus-specific pan-<jats:italic>Bartonella<\/jats:italic>FRET-qPCR. Then, a<jats:italic>gltA<\/jats:italic>-based<jats:italic>Bartonella<\/jats:italic>PCR was established by selecting primers for a highly variable region of<jats:italic>gltA<\/jats:italic>, of which the sequenced amplicons could identify individual<jats:italic>Bartonella<\/jats:italic>spp. The pan-<jats:italic>Bartonella<\/jats:italic>FRET-qPCR did not detect negative controls (<jats:italic>Brucella<\/jats:italic>spp.,<jats:italic>Anaplasma<\/jats:italic>spp.,<jats:italic>Rickettsia<\/jats:italic>spp.,<jats:italic>Coxiella burnetii<\/jats:italic>, and<jats:italic>Wolbachia<\/jats:italic>) but reliably detected as few as two copies of the positive control (<jats:italic>Bartonella henselae<\/jats:italic>) per reaction. There was complete agreement between the pan-<jats:italic>Bartonella<\/jats:italic>FRET-qPCR and the<jats:italic>gltA<\/jats:italic>-based<jats:italic>Bartonella<\/jats:italic>PCR in detecting<jats:italic>Bartonella<\/jats:italic>in convenience test samples from China and St. Kitts: cats (26%; 81\/310),<jats:italic>Ctenocephalides felis<\/jats:italic>(20%; 12\/60), cattle (24%; 23\/98), and donkeys (4%; 1\/20). Sequencing of the<jats:italic>gltA<\/jats:italic>-based<jats:italic>Bartonella<\/jats:italic>PCR products revealed<jats:italic>B. henselae<\/jats:italic>(70%; 57\/81) and<jats:italic>B. clarridgeiae<\/jats:italic>(30%; 24\/81) in cats and<jats:italic>C. felis<\/jats:italic>(67%; 8\/12, and 33%; 4\/12, respectively) and<jats:italic>B. bovis<\/jats:italic>in cattle (23.5%; 23\/98) and donkeys (4.0%; 1\/24). The pan-<jats:italic>Bartonella<\/jats:italic>FRET-qPCR and<jats:italic>gltA<\/jats:italic>-based<jats:italic>Bartonella<\/jats:italic>PCR we developed are highly sensitive and specific in detecting recognized<jats:italic>Bartonella<\/jats:italic>spp. in a single reaction. The pan-<jats:italic>Bartonella<\/jats:italic>FRET-qPCR is convenient requiring no gel electrophoresis and providing copy numbers, while the<jats:italic>gltA<\/jats:italic>-based<jats:italic>Bartonella<\/jats:italic>PCR reliably differentiates individual<jats:italic>Bartonella<\/jats:italic>species. The use of these PCRs should greatly facilitate large-scale surveillance studies and the diagnosis of infections in clinical samples.<\/jats:p>","DOI":"10.1155\/2019\/3209013","type":"journal-article","created":{"date-parts":[[2019,9,3]],"date-time":"2019-09-03T23:30:38Z","timestamp":1567553438000},"page":"1-9","source":"Crossref","is-referenced-by-count":14,"title":["Molecular Detection of<i>Bartonella<\/i>spp. in China and St. Kitts"],"prefix":"10.1155","volume":"2019","author":[{"given":"Ke","family":"Huang","sequence":"first","affiliation":[{"name":"Yangzhou University College of Veterinary Medicine, Yangzhou, Jiangsu 225009, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick John","family":"Kelly","sequence":"additional","affiliation":[{"name":"Ross University School of Veterinary Medicine, Basseterre, Saint Kitts and 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