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To investigate the immune regulation mechanism of herb-partitioned moxibustion in rats with Crohn\u2019s disease (CD) focusing on autophagy.<jats:italic> Methods<\/jats:italic>. Rats were randomly divided into normal (N) group, CD model (M) group, CD model with herb-partitioned moxibustion (MM) group, normal with herb-partitioned moxibustion (NM) group, CD model with mesalazine (western medicine, Med ) group, and normal saline (NS) group, with 10 rats in each group. The CD model rats were prepared by trinitrobenzene sulphonic expect for the N group and NM group. After the CD rats model were established, the rats in the MM and NM groups were treated with herb-partitioned moxibustion at Tianshu (ST25) and Qihai (CV6) acupoints once daily for 7 days, and rats in the Med and NS groups were respectively treated with mesalazine enteric coated tablet and normal saline once daily for 7 days. After intervention, hematoxylin-eosin staining was used to observe the histological changes of colon; RNA sequencing was used to observe the changes in autophagy- and immune-associated gene expression profiles. In addition, autophagy- and immune-associated cytokines and signaling pathways in CD rats were also screened.<jats:italic> Results<\/jats:italic>. HPM significantly increased the body weight of CD rats (<jats:italic>P<\/jats:italic>&lt;0.01) and improved the pathological injury of colon in CD rats (<jats:italic>P<\/jats:italic>&lt;0.01). HPM also changed the expression of many autophagy- and immune-associated genes, especially downregulating the expression of autophagy-associated<jats:italic> Nod2<\/jats:italic>,<jats:italic> Irgm<\/jats:italic> genes as well as the receptor of immune-associated<jats:italic> Il12b<\/jats:italic>,<jats:italic> Il22 <\/jats:italic>(<jats:italic>Il12rb1<\/jats:italic>,<jats:italic> Il22ra2<\/jats:italic>) genes in the colon of CD rats. HPM also changed the enrichment levels of differentially expressed genes in the human T-cell leukemia virus type-1 infection pathway, the Epstein-Barr virus infection pathway, and the cell adhesion molecule pathway. In addition, the expression levels of Nod2, Irgm, IL-12b, and IL-22 mRNA were increased (all<jats:italic> P<\/jats:italic>&lt; 0.01) in the M group compared to the N group, while the expression levels of Nod2, Irgm, IL-12b, and IL-22 mRNA were decreased (<jats:italic>P<\/jats:italic>&lt;0.05 or<jats:italic> P<\/jats:italic>&lt;0.01) in the MM and Med groups compared to the M group.<jats:italic> Conclusion<\/jats:italic>. Herb-partitioned moxibustion may effectively attenuate intestinal inflammation and promote the repair of colon mucosal injury of CD rats through the regulation of autophagy- and immune-associated gene expression and signaling pathways.<\/jats:p>","DOI":"10.1155\/2019\/3405146","type":"journal-article","created":{"date-parts":[[2019,3,7]],"date-time":"2019-03-07T23:31:26Z","timestamp":1552001486000},"page":"1-13","source":"Crossref","is-referenced-by-count":10,"title":["Effect of Herb-Partitioned Moxibustion on Autophagy and Immune-Associated Gene Expression Profiles in a Rat Model of Crohn\u2019s Disease"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9075-6036","authenticated-orcid":true,"given":"Ji-meng","family":"Zhao","sequence":"first","affiliation":[{"name":"Key Laboratory of Acupuncture and Immunological Effects, Shanghai Research Institute of Acupuncture and Meridian, Shanghai University of Traditional Chinese Medicine, Shanghai 200030, 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