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Rat renal proximal tubular epithelial cells (NRK-52E) were exposed to various concentrations of uric acid in the presence or absence of telmisartan. Treatment with uric acid increased the expression of<jats:italic>\u03b1<\/jats:italic>-SMA, decreased the expression of E-cadherin, and promoted EMT in NRK-52E cells. Uric acid treatment also led to increased endothelin-1 (ET-1) production, activation of extracellular-regulated protein kinase 1\/2 (ERK1\/2), and the upregulation of nicotinamide adenine dinucleotide phosphate oxidase 4 (NOX4). Use of ET-1 receptor inhibitor (BQ123 or BQ788) could inhibit uric acid-induced EMT in NRK-52E cells. Pretreatment with the ERK inhibitor (U0126 or PD98059) suppressed the release of ET-1 and EMT induced by uric acid. Additionally, pretreatment with a traditional antioxidant (diphenylene iodonium or apocynin) inhibited the activation of ERK1\/2, release of ET-1, and uric acid-induced EMT in NRK-52E cells. These findings suggested that uric acid-induced EMT in renal tubular epithelial cells occurs through NADPH oxidase-mediated ERK1\/2 activation and the subsequent release of ET-1. Furthermore, telmisartan (10<jats:sup>2<\/jats:sup>nmol\/L to 10<jats:sup>4<\/jats:sup>nmol\/L) inhibited the expression of NOX4, intracellular reactive oxygen species (ROS), activation of ERK1\/2, and the release of ET-1 in a dose-dependent manner, thereby preventing uric acid-induced EMT in NRK-52E. In conclusion, telmisartan could ameliorate uric acid-induced EMT in NRK-52E cells likely through inhibition of the NADPH oxidase\/ERK1\/2\/ET-1 pathway.<\/jats:p>","DOI":"10.1155\/2019\/3851718","type":"journal-article","created":{"date-parts":[[2019,3,12]],"date-time":"2019-03-12T23:30:50Z","timestamp":1552433450000},"page":"1-12","source":"Crossref","is-referenced-by-count":23,"title":["Telmisartan Attenuates Uric Acid-Induced Epithelial-Mesenchymal Transition in Renal Tubular Cells"],"prefix":"10.1155","volume":"2019","author":[{"given":"Dongqing","family":"Zha","sequence":"first","affiliation":[{"name":"Division of Nephrology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430070, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saiqun","family":"Wu","sequence":"additional","affiliation":[{"name":"Division of Nephrology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430070, 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