{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:43:20Z","timestamp":1791254600931,"version":"4.1.0"},"reference-count":79,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T00:00:00Z","timestamp":1550448000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007235","name":"Colorado State University","doi-asserted-by":"publisher","award":["#5T32OD012201-05"],"award-info":[{"award-number":["#5T32OD012201-05"]}],"id":[{"id":"10.13039\/100007235","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["#5T32OD012201-05"],"award-info":[{"award-number":["#5T32OD012201-05"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Environmental and Public Health"],"published-print":{"date-parts":[[2019,2,18]]},"abstract":"<jats:p>Waterborne<jats:italic>Escherichia coli<\/jats:italic>are a major reservoir of antimicrobial resistance (AMR), including but not limited to extended-spectrum beta-lactamase (ESBL) and<jats:italic>Klebsiella pneumoniae<\/jats:italic>carbapenemase (KPC) mechanisms. This study quantified and described ESBL- and KPC-producing<jats:italic>E. coli<\/jats:italic>in Northern Colorado from sewer water, surface water, and influent and effluent wastewater treatment sources. Total detected bacteria and<jats:italic>E. coli<\/jats:italic>abundances, and the percentages that contain ESBL and\/or KPC, were compared between water sources. Seventy<jats:italic>E. coli<\/jats:italic>isolates from the various waters had drug resistance validated with a panel of 17 antibiotics using a broth microdilution assay. The diverse drug resistance observed across<jats:italic>E. coli<\/jats:italic>isolates was further documented by polymerase chain reaction of common ESBL genes and functional relatedness by PhenePlate assay-generated dendrograms (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>70<\/mml:mn><\/mml:mrow><\/mml:math>). The total<jats:italic>E. coli<\/jats:italic>abundance decreased through the water treatment process as expected, yet the percentages of<jats:italic>E. coli<\/jats:italic>harboring ESBL resistance were increased (1.70%) in surface water. Whole-genome sequencing analysis was completed for 185 AMR genes in wastewater<jats:italic>E. coli<\/jats:italic>isolates and confirmed the presence of diverse AMR gene classes (e.g., beta-lactams and efflux pumps) in isolate genomes. This study completed surveillance of AMR patterns in<jats:italic>E. coli<\/jats:italic>that reside in environmental water systems and suggests a role for integrating both phenotypic and genotypic profiling beyond ESBL and KPC mechanisms. AMR screening via multiple approaches may assist in the prevention of drug-resistant<jats:italic>E. coli<\/jats:italic>spread from waters to animals and humans.<\/jats:p>","DOI":"10.1155\/2019\/3862949","type":"journal-article","created":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T23:31:27Z","timestamp":1550532687000},"page":"1-13","source":"Crossref","is-referenced-by-count":61,"title":["Antimicrobial-Resistant<i>Escherichia coli<\/i>from Environmental Waters in Northern Colorado"],"prefix":"10.1155","volume":"2019","author":[{"given":"Hannah B.","family":"Haberecht","sequence":"first","affiliation":[{"name":"Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nora Jean","family":"Nealon","sequence":"additional","affiliation":[{"name":"Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523, USA"},{"name":"Graduate 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