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This paper focuses on the Hopf bifurcation in imitative dynamics of three strategies (Rock\u2010Paper\u2010Scissors) with mutations. First, we verify that there is a Hopf bifurcation in the imitative dynamics with no mutation. Then, we find that there is a critical value of mutation such that the system tends to an unstable limit cycle created in a subcritical Hopf bifurcation. Moreover, the Hopf bifurcation exists for other kinds of the considered mutation patterns. 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