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Thus, the primary aim of the present study was to determine whether glycine could (1) attenuate HIE injury in rats and hypoxic stress in PC12 cells and (2) downregulate mitochondria-mediated autophagy dependent on the adenosine monophosphate- (AMP-) activated protein kinase (AMPK) pathway. Experiments conducted using an <jats:italic>in vivo<\/jats:italic> HIE animal model and <jats:italic>in vitro<\/jats:italic> hypoxic stress to PC12 cells revealed that intense autophagy associated with mitochondrial function occurred during <jats:italic>in vivo<\/jats:italic> HIE injury and <jats:italic>in vitro<\/jats:italic> hypoxic stress. However, glycine treatment effectively attenuated mitochondria-mediated autophagy. Additionally, after identifying alterations in proteins within the AMPK pathway in rats and PC12 cells following glycine treatment, cyclosporin A (CsA) and 5-aminoimidazole-4-carboxamide-1-b-4-ribofuranoside (AICAR) were administered in these models and indicated that glycine protected against HIE and CoCl<jats:sub>2<\/jats:sub> injury by downregulating mitochondria-mediated autophagy that was dependent on the AMPK pathway. Overall, glycine attenuated hypoxic-ischemic injury in neurons via reductions in mitochondria-mediated autophagy through the AMPK pathway both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2019\/4248529","type":"journal-article","created":{"date-parts":[[2019,2,6]],"date-time":"2019-02-06T18:30:58Z","timestamp":1549477858000},"page":"1-29","source":"Crossref","is-referenced-by-count":46,"title":["Glycine Protects against Hypoxic-Ischemic Brain Injury by Regulating Mitochondria-Mediated Autophagy via the AMPK Pathway"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3800-3971","authenticated-orcid":true,"given":"Chen-chen","family":"Cai","sequence":"first","affiliation":[{"name":"Department of Neonatology, The Second Affiliated Hospital and Yuying Children\u2019s Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325027, 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