{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T09:14:37Z","timestamp":1777454077850,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T00:00:00Z","timestamp":1555459200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["246595"],"award-info":[{"award-number":["246595"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003069","name":"Instituto Polit\u00e9cnico Nacional","doi-asserted-by":"publisher","award":["246595"],"award-info":[{"award-number":["246595"]}],"id":[{"id":"10.13039\/501100003069","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Sensors"],"published-print":{"date-parts":[[2019,4,17]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Obesity in children is highly prevalent in Mexican population. Adipose tissue has been related to specific pro- and anti-inflammatory cytokine and inflammasome gene and protein expression patterns. Actually, there is no existing biosensor for detecting gene expression patterns in children with obesity. The quartz crystal microbalance with dissipation monitoring (QCM-D) has been used as a transducer for DNA biosensor design. <jats:italic>Results<\/jats:italic>. In this study, the gene expression pattern of <jats:italic>IL-1\u03b2<\/jats:italic>, <jats:italic>NLRP3<\/jats:italic>, and <jats:italic>CASPASE-1<\/jats:italic> in children with obesity was successfully determined by means of QCM-D. Gene expression patterns were validated with those obtained by quantitative polymerase chain reaction (qPCR), a validated molecular biology technique for gene expression quantification. QCM-D analysis of the detected mass corresponding results for each of the genes showed a major detected mass for <jats:italic>IL-1\u03b2<\/jats:italic>, followed by similar <jats:italic>NLRP3<\/jats:italic> and constitutive gene <jats:italic>18S<\/jats:italic> deposited mass and a smaller deposited mass for <jats:italic>CASPASE-1<\/jats:italic>. Surprisingly, when comparing mRNA gene expression results for <jats:italic>NLRP3<\/jats:italic>, <jats:italic>IL-1\u03b2<\/jats:italic>, and <jats:italic>CASPASE-1<\/jats:italic> obtained with qPCR and QCM-D, similar patterns were found, revealing greatest expression of <jats:italic>IL-1\u03b2<\/jats:italic>, followed by <jats:italic>NLRP3<\/jats:italic>, with <jats:italic>CASPASE-1<\/jats:italic> being the molecule of least expression in the group of children with obesity. AFM images illustrate the step-by-step changes that took place on the quartz surface. <jats:italic>Conclusions<\/jats:italic>. QCM-D proved successfully for determining the gene transcripts and expression of <jats:italic>NLRP3<\/jats:italic>, <jats:italic>IL-1\u03b2<\/jats:italic>, and <jats:italic>CASPASE-1<\/jats:italic> in children with obesity, with similar results validated by qPCR. \u201cQCM-D decreases detection costs compared with a validated molecular biology technique.\u201d The QCM-D biosensor developed by our group was successful for gene expression determination; in the future, it can be used for molecular diagnosis.<\/jats:p>","DOI":"10.1155\/2019\/4323056","type":"journal-article","created":{"date-parts":[[2019,4,17]],"date-time":"2019-04-17T20:01:20Z","timestamp":1555531280000},"page":"1-14","source":"Crossref","is-referenced-by-count":4,"title":["<i>NLRP3<\/i>, <i>IL-1\u03b2<\/i>, and <i>Caspase-1<\/i> Gene Transcript Identification and Expression by QCM-D in Obese Children"],"prefix":"10.1155","volume":"2019","author":[{"given":"Modesto","family":"G\u00f3mez L\u00f3pez","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y D\u00edaz Mir\u00f3n, C.P. 11340 Ciudad de Mexico, Mexico"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9691-9035","authenticated-orcid":true,"given":"Nadia Mabel","family":"P\u00e9rez-Vielma","sequence":"additional","affiliation":[{"name":"Centro Interdisciplinario de Ciencias de la Salud Unidad Santo Tom\u00e1s, Instituto Polit\u00e9cnico Nacional, Avenida de los Maestros, C.P. 11340 Ciudad de Mexico, Mexico"}]},{"given":"Haydee","family":"Gonz\u00e1lez Mart\u00ednez","sequence":"additional","affiliation":[{"name":"Centro de Nanociencias y Micro y Nanotecnolog\u00edas, Instituto Polit\u00e9cnico Nacional, Av. Luis Enrique Erro, C.P. 07738 Ciudad de Mexico, Mexico"}]},{"given":"Eleazar","family":"Lara Padilla","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y D\u00edaz Mir\u00f3n, C.P. 11340 Ciudad de Mexico, Mexico"}]},{"given":"Cindy","family":"Bandala","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Estudios de Posgrado e Investigaci\u00f3n, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y D\u00edaz Mir\u00f3n, C.P. 11340 Ciudad de Mexico, Mexico"}]},{"given":"Mar\u00eda Cristina","family":"Gonz\u00e1lez Torres","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Celular, Universidad Aut\u00f3noma Metropolitana-Iztapalapa, Av. San Rafael Atlixco, C.P.09340 Ciudad de Mexico, Mexico"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3958-0869","authenticated-orcid":true,"given":"\u00c1ngel","family":"Miliar-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular, Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Plan de San Luis y D\u00edaz Mir\u00f3n, C.P. 11340 Ciudad de Mexico, Mexico"},{"name":"Centro Interdisciplinario de Ciencias de la Salud Unidad Santo Tom\u00e1s, Instituto Polit\u00e9cnico Nacional, Avenida de los Maestros, C.P. 11340 Ciudad de Mexico, 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