{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T22:58:36Z","timestamp":1773788316837,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T00:00:00Z","timestamp":1547683200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Planned Projects of Jinhua City","award":["Y20153004"],"award-info":[{"award-number":["Y20153004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2019,1,17]]},"abstract":"<jats:p><jats:italic>Aim<\/jats:italic>. To explore the therapeutic effects and mechanisms of interleukin 10 gene-modified bone marrow-derived dendritic cells (DC-IL10) on liver fibrosis. <jats:italic>Methods<\/jats:italic>. In vitro, BMDCs were transfected with lentiviral-interleukin 10-GFP (LV-IL10-GFP) at the MOI of 1\u2009:\u200940. Then, the phenotype (MHCII, CD80, and CD86) and allo-stimulatory ability of DC-IL10 were identified by flow cytometry, and the levels of IL-10 and IL-12 (p70) secreted into the culture supernatants were quantified by ELISA. In vivo, DC-IL10 was injected into mice with CCl4-induced liver fibrosis through the tail vein. Lymphocytes were isolated to investigate the differentiation of T cells, and serum and liver tissue were collected for biochemical, cytokine, histopathologic, immune-histochemical, and Western blot analyzes. <jats:italic>Results<\/jats:italic>. In vitro, the expressions of MHCII, CD80, and CD86 in DC-IL10 were significantly suppressed, allogeneic CD4<jats:sup>+<\/jats:sup>T cells incubated with DC-IL10 showed a lower proliferative response, and the levels of IL-10 and IL-12 (p70) secreted into the DC-IL10 culture supernatants were significantly increased and decreased, respectively. In vivo, regulatory T cells (Tregs) were significantly increased, while ALT, AST, and inflammatory cytokines were significantly reduced in the DC-IL10 treatment group, and the degree of hepatic fibrosis was obviously reversed. The TGF-<jats:italic>\u03b2<\/jats:italic>\/smad pathway was inhibited following DC-IL10 treatment compared to the liver fibrosis group. <jats:italic>Conclusion<\/jats:italic>. IL-10 genetic modification of BMDCs may maintain DC in the state of tolerance and allow DC to induce T cell hyporesponsiveness or tolerance. DC-IL10 suppressed liver fibrosis by inducing Treg production and inhibiting the TGF-<jats:italic>\u03b2<\/jats:italic>\/smad signaling pathway.<\/jats:p>","DOI":"10.1155\/2019\/4652596","type":"journal-article","created":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T18:39:22Z","timestamp":1547750362000},"page":"1-15","source":"Crossref","is-referenced-by-count":35,"title":["Interleukin 10 Gene-Modified Bone Marrow-Derived Dendritic Cells Attenuate Liver Fibrosis in Mice by Inducing Regulatory T Cells and Inhibiting the TGF-<i>\u03b2<\/i>\/Smad Signaling Pathway"],"prefix":"10.1155","volume":"2019","author":[{"given":"Yejin","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, Jinhua Municipal Central Hospital, Jinhua, Zhejiang, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyue","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, the First Affiliated Hospital of Gannan 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