{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T07:09:03Z","timestamp":1780556943475,"version":"3.54.1"},"reference-count":25,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,5,2]],"date-time":"2019-05-02T00:00:00Z","timestamp":1556755200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Sensors"],"published-print":{"date-parts":[[2019,5,2]]},"abstract":"<jats:p>In this study, we integrated genetic detection for polymerase chain reaction (PCR) with microfluidics technology for the detection of peanut DNA. A cross-junction microchannel was used to induce emulsion droplets of water in oil for PCR on a chip. Compared with the single-phase flow, the emulsion droplet flow exhibited a 7.24% lower evaporation amount and prevented air bubble generation. PCR results of the droplet microfluidic PCR chip for peanut DNA fragment detection was verified by comparison with a commercial PCR thermal cycler and increased fluorescence intensity in SYBR Green reagent-based PCR. Moreover, PCR on the microfluidic PCR chip was successful for sesame, <jats:italic>Salmonella<\/jats:italic> spp., and <jats:italic>Staphylococcus aureus<\/jats:italic>. The droplet microfluidic PCR device developed in this study can be applied for peanut detection in the context of food allergy.<\/jats:p>","DOI":"10.1155\/2019\/4712084","type":"journal-article","created":{"date-parts":[[2019,5,2]],"date-time":"2019-05-02T19:34:50Z","timestamp":1556825690000},"page":"1-9","source":"Crossref","is-referenced-by-count":23,"title":["Peanut Detection Using Droplet Microfluidic Polymerase Chain Reaction Device"],"prefix":"10.1155","volume":"2019","author":[{"given":"Shou-Yu","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering, National United University, Miaoli, Taiwan"},{"name":"Ph.D. Program in Tissue Engineering and Regenerative Medicine, National Chung Hsing University, Taichung, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Cheng","family":"Chiang","sequence":"additional","affiliation":[{"name":"Department of Food Nutrition and Health Biotechnology, Asia University, Taichung, 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