{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T12:16:34Z","timestamp":1788524194987,"version":"build-2803163510"},"reference-count":42,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,12,28]],"date-time":"2019-12-28T00:00:00Z","timestamp":1577491200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002660","name":"Islamic Azad University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002660","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2019,12,28]]},"abstract":"<jats:p><jats:italic>Introduction<\/jats:italic>. Wortmannin (WTN) is a steroid metabolite that inhibits phosphatidylinositol 3-kinase and other signaling pathways. Structurally, the WTN consists of a cyclopentanophenanthrene-like structure with several oxygen-rich moieties which have the potential to interact with deoxyribonucleic acid (DNA) molecules.<jats:italic>Methods<\/jats:italic>. We aim to evaluate the WTN and calf thymus DNA (ct-DNA) interaction with molecular docking using the AutoDock 4.2 software. UV and fluorescence spectroscopy and viscosity techniques were performed to confirm the<jats:italic>in silico<\/jats:italic>analysis.<jats:italic>Results<\/jats:italic>. Molecular docking showed that the WTN interacted with ct-DNA via hydrogen bonds at guanine-rich sequences. The number of hydrogen bonds between the WTN and DNA was 1-2 bonds (average 1.2) per WTN molecule. The<jats:italic>in silico<\/jats:italic>binding constant was 2\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>\u2009M<jats:sup>\u22121<\/jats:sup>. UV spectroscopy showed that the WTN induced a hyperchromic feature without wavelength shifting. The WTN and DNA interaction led to quenching of DNA-emitted fluorescence. The different concentrations of WTN had no effect on DNA viscosity. Taken together, our results demonstrated WTN interacts with DNA in the nonintercalating mode, which is considered as a new mechanism of action.<jats:italic>Conclusion<\/jats:italic>. These results suggest that the WTN may exert its biological effects, at least in part, via interaction with DNA.<\/jats:p>","DOI":"10.1155\/2019\/4936351","type":"journal-article","created":{"date-parts":[[2019,12,28]],"date-time":"2019-12-28T23:31:01Z","timestamp":1577575861000},"page":"1-7","source":"Crossref","is-referenced-by-count":12,"title":["Study of the Binding Interaction between Wortmannin and Calf Thymus DNA: Multispectroscopic and Molecular Docking Studies"],"prefix":"10.1155","volume":"2019","author":[{"given":"Shiva","family":"Mehran","sequence":"first","affiliation":[{"name":"Department of Biology, Higher Education Institute of Rabe-Rashidi, Tabriz, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yousef","family":"Rasmi","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Urmia University of Medical Science, Urmia, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid 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