{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T04:40:24Z","timestamp":1790052024634,"version":"4.0.1"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T00:00:00Z","timestamp":1560729600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011093","name":"University of Phayao","doi-asserted-by":"publisher","award":["R020058217046"],"award-info":[{"award-number":["R020058217046"]}],"id":[{"id":"10.13039\/501100011093","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2019,6,17]]},"abstract":"<jats:p><jats:italic>Salmonella enterica<\/jats:italic>Serotype 4,[5],12:i:-, a monophasic variant of<jats:italic>S<\/jats:italic>. Typhimurium, with high virulence and multidrug resistance is distributed globally causing pathogenicity to both humans and domesticated animals. BOX-A1R-based repetitive extragenic palindromic-PCR (BOX)-PCR proved to be superior to three other repetitive element-based PCR typing methods, namely, enterobacterial repetitive intergenic consensus (ERIC)-, poly-trinucleotide (GTG)<jats:sub>5<\/jats:sub>-, and repetitive extragenic palindromic (REP)-PCR (carried out under a single optimized amplification condition), in differentiating genetic relatedness among<jats:italic>S<\/jats:italic>. 4,[5],12:i:- isolates from feces of hospitalized patients (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>12<\/mml:mn><\/mml:mrow><\/mml:math>) and isolates from minced pork samples of<jats:italic>S<\/jats:italic>. 4,[5],12:i:- (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>6<\/mml:mn><\/mml:mrow><\/mml:math>),<jats:italic>S<\/jats:italic>. Typhimurium (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>6<\/mml:mn><\/mml:mrow><\/mml:math>), and<jats:italic>Salmonella<\/jats:italic>Serogroup B (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>4<\/mml:mn><\/mml:mrow><\/mml:math>) collected from different regions of northern Thailand. Construction of phylogenetic trees from amplicon size patterns allowed allocation of<jats:italic>Salmonella<\/jats:italic>isolates into clusters of similar genetic relatedness, with BOX-PCR generating more unique clusters for each serotype than the other three typing methods. BOX-, (GTG)<jats:sub>5<\/jats:sub>-, and REP-PCR indicated significant genetic relatedness between<jats:italic>S<\/jats:italic>. 4,[5],12:i:- isolates 1 and 9 from hospitalized patients and<jats:italic>S<\/jats:italic>. 4,[5],12:i:- isolate en 29 from minced pork, suggesting a possible route of transmission. Thus, BOX-PCR provides a suitable molecular typing method for discriminating genetic relatedness among<jats:italic>Salmonella<\/jats:italic>spp. of the same and different serotypes and should be suitable for application in typing and tracking route of transmission in<jats:italic>Salmonella<\/jats:italic>outbreaks.<\/jats:p>","DOI":"10.1155\/2019\/5086240","type":"journal-article","created":{"date-parts":[[2019,6,17]],"date-time":"2019-06-17T23:31:33Z","timestamp":1560814293000},"page":"1-11","source":"Crossref","is-referenced-by-count":15,"title":["Effectiveness of BOX-PCR in Differentiating Genetic Relatedness among<i>Salmonella enterica<\/i>Serotype 4,[5],12:i:- Isolates from Hospitalized Patients and Minced Pork Samples in Northern Thailand"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6517-4755","authenticated-orcid":true,"given":"Kritchai","family":"Poonchareon","sequence":"first","affiliation":[{"name":"Division of Biochemistry, School of 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