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Here, we analysed two different types of tissue microarrays of healthy lymphatic and various inflamed tissues, colorectal and lung cancers, as well as &gt;1700 tumour samples from 86 different tumour entities for TIGIT and\/or PD-1 by bright field and\/or multiplex fluorescence immunohistochemistry. TIGIT was detected in CD8<jats:sup>+<\/jats:sup> cytotoxic T cells, CD4<jats:sup>+<\/jats:sup> T helper cells, FOXP3<jats:sup>+<\/jats:sup> regulatory T cells, and NK cells, but not in CD11c<jats:sup>+<\/jats:sup> dendritic cells, CD68<jats:sup>+<\/jats:sup> macrophages, and CD20<jats:sup>+<\/jats:sup> B lymphocytes. TIGIT expression paralleled that of PD-1. More than 70% of TIGIT<jats:sup>+<\/jats:sup> cells were PD-1<jats:sup>+<\/jats:sup>, and more than 90% of the PD-1<jats:sup>+<\/jats:sup> cells were TIGIT<jats:sup>+<\/jats:sup>. Expression varied between different tissue compartments. TIGIT expression in tonsil gradually increased from the interfollicular area over the marginal\/mantle zone to the germinal centre in all T cell subtypes. In inflammatory diseases, the strongest expression of TIGIT\/PD-1 was found in Hashimoto thyroiditis. TIGIT<jats:sup>+<\/jats:sup> lymphocytes were seen in all 86 different tumour entities with considerable high variability of TIGIT positivity within and between different cancer entities. Particularly, high densities of TIGIT<jats:sup>+<\/jats:sup> lymphocytes were, for example, seen in squamous cell cancers of various origins. In summary, the variable expression levels of TIGIT and PD-1 in cell types and tissue compartments illustrate the high complexity of immune microenvironments. The high frequency of TIGIT (and PD-1) expressing lymphocytes in cancers highlights considerable opportunities for cotargeting with checkpoint inhibitors.<\/jats:p>","DOI":"10.1155\/2019\/5160565","type":"journal-article","created":{"date-parts":[[2019,1,10]],"date-time":"2019-01-10T18:32:19Z","timestamp":1547145139000},"page":"1-13","source":"Crossref","is-referenced-by-count":67,"title":["Patterns of TIGIT Expression in Lymphatic Tissue, Inflammation, and Cancer"],"prefix":"10.1155","volume":"2019","author":[{"given":"Niclas C.","family":"Blessin","sequence":"first","affiliation":[{"name":"Institute of Pathology, University Medical Centre Hamburg-Eppendorf, 20246 Hamburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0158-4258","authenticated-orcid":true,"given":"Ronald","family":"Simon","sequence":"additional","affiliation":[{"name":"Institute of Pathology, University Medical Centre Hamburg-Eppendorf, 20246 Hamburg, 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