{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T22:28:14Z","timestamp":1777415294111,"version":"3.51.4"},"reference-count":62,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,3,14]],"date-time":"2019-03-14T00:00:00Z","timestamp":1552521600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2019,3,14]]},"abstract":"<jats:p><jats:italic>Introduction<\/jats:italic>. The acute kidney injury (AKI) is characterized by a sudden glomerular filtration reduction. Renal or intrinsic causes of AKI include nephrotoxicity induced by exogenous agents like cisplatin, which causes oxidative stress altering the biochemical process and leading to apoptosis. Therefore, this research is aimed at analyzing the embryonic stem cells (ESC) nephroprotective effect in AKI induced by cisplatin, employing genetic, phenotypic, and microspectroscopic techniques.<jats:italic>Methods<\/jats:italic>. Thirty mice were randomly divided into three groups (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>10<\/mml:mn><\/mml:math>): the healthy, isotonic salt solution (ISS), and mouse embryonic stem cells (mESC) groups. The ISS and mESC groups were subjected to AKI using cisplatin; 24\u2009h post-AKI received an intraperitoneal injection of ISS or<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mn>1<\/mml:mn><mml:mo>\u00d7<\/mml:mo><mml:msup><mml:mrow><mml:mn>10<\/mml:mn><\/mml:mrow><mml:mrow><mml:mn>6<\/mml:mn><\/mml:mrow><\/mml:msup><\/mml:math>mESC, respectively. At days 4 and 8 post-AKI, five mice of each group were sacrificed to analyze the histopathological, genetic (<jats:italic>PDK4<\/jats:italic>and<jats:italic>HO-1<\/jats:italic>), protein (p53), and vibrational microspectroscopic changes.<jats:italic>Results.<\/jats:italic>Histopathologically, interstitial nephritis and acute tubular necrosis were observed; however, the mESC group showed a more preserved microarchitecture with high cellularity. Additionally, the PDK4 and HO-1 gene expression only increased in the ISS group on day 4 post-AKI. Likewise, p53 was more immunoexpressed at day 8 post-AKI in the ISS group. About biomolecular analysis by microspectroscopy, bands associated with lipids, proteins, and nucleic acids were evidenced. Besides, ratios related to membrane function (protein\/lipid), unsaturated lipid content (olefinic\/total lipid, olefinic\/total CH<jats:sub>2<\/jats:sub>, and CH<jats:sub>2<\/jats:sub>\/CH<jats:sub>3<\/jats:sub>), and lipid peroxidation demonstrated oxidative stress induction and lipid peroxidation increase mainly in the ISS group. Finally, the principal component analysis discriminated against each group; nonetheless, some data of the healthy and mESC groups at day 8 were correlated.<jats:italic>Conclusions<\/jats:italic>. The mESC implant diminishes cisplatin nephrotoxicity, once the protective effect in the reduction of lipid peroxidation was demonstrated, reflecting a functional and histological restoration.<\/jats:p>","DOI":"10.1155\/2019\/5420624","type":"journal-article","created":{"date-parts":[[2019,3,14]],"date-time":"2019-03-14T23:34:11Z","timestamp":1552606451000},"page":"1-14","source":"Crossref","is-referenced-by-count":13,"title":["Nephroprotective Effect of Embryonic Stem Cells Reducing Lipid Peroxidation in Kidney Injury Induced by Cisplatin"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1811-4511","authenticated-orcid":true,"given":"Monica Maribel","family":"Mata-Miranda","sequence":"first","affiliation":[{"name":"Escuela Militar de Medicina, Centro Militar de Ciencias de la Salud, Secretaria de la Defensa Nacional, Ciudad de M\u00e9xico 11200, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos 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