{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T22:57:43Z","timestamp":1770332263898,"version":"3.49.0"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T00:00:00Z","timestamp":1575504000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2019,12,5]]},"abstract":"<jats:p>A new method for the detection by flow cytometry of anti-double-stranded DNA antibodies and of circulating immune complexes (IC) containing endogenous DNA (IC-eDNA) is described. From each serum sample, two samples were taken, one was used to detect IC-eDNA. The other to detect anti-DNA antibodies was incubated with calf thymus DNA. ICs were isolated by polyethylene glycol precipitation or by cryoprecipitation, after which immunoglobulins were labeled with FITC-conjugated anti-human globulin. Serum samples from 63 systemic lupus erythematosus (SLE) patients, 32 incomplete lupus, and 87 control patients were tested. Detection of anti-dsDNA antibodies by flow cytometry had a diagnostic sensitivity and specificity almost comparable to routine tests, the fluorescent enzyme immunoassay EliA\u2122-dsDNA test, and the ultrasensitive Crithidia luciliae indirect immunofluorescence test. In 21 (33%) out of 63 SLE serum samples, IC-eDNA was detected. In these samples, free anti-dsDNA antibodies were hardly detectable or undetectable by flow cytometry or by routine tests. When anti-DNA antibodies are neutralized by endogenous DNA and can no longer be detected by routine tests, the serologic diagnosis and the follow-up of relapses in patients with SLE is compromised. To overcome this obstacle, we propose an accessible solution: the detection of circulating IC-eDNA by flow cytometry.<\/jats:p>","DOI":"10.1155\/2019\/6047085","type":"journal-article","created":{"date-parts":[[2019,12,5]],"date-time":"2019-12-05T23:31:18Z","timestamp":1575588678000},"page":"1-12","source":"Crossref","is-referenced-by-count":8,"title":["Detection by Flow Cytometry of Anti-DNA Autoantibodies and Circulating DNA Immune Complexes in Lupus Erythematosus"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1277-0593","authenticated-orcid":true,"given":"Nisen","family":"Abuaf","sequence":"first","affiliation":[{"name":"Laboratoire d\u2019H\u00e9matologie et d\u2019Immunologie, H\u00f4pital Tenon, Groupe Hospitalier Universitaire Paris Est, AP-HP et D\u00e9partement d\u2019Immunologie, Universit\u00e9 Pierre et Marie Curie, 4 Rue de la Chine, 75020 Paris, France"},{"name":"Service de Dermatologie et d\u2019Allergie, H\u00f4pital Tenon, 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