{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:39:40Z","timestamp":1791841180490,"version":"4.3.4"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T00:00:00Z","timestamp":1550448000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Pan African University"},{"name":"Institute for Basic Sciences, Technology and Innovation"},{"name":"Africa-ai-Japan Project"},{"DOI":"10.13039\/501100009402","name":"Mekelle University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100009402","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2019,2,18]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. This study has investigated the antimicrobial activity of extracts of indigenous wild mushrooms against selected organisms.<jats:italic> Methods<\/jats:italic>. Thirty-five (35) indigenous wild mushrooms were collected from Arabuko-Sokoke and Kakamega National Reserve Forests, Kenya. All mushrooms were identified and their contents were extracted and screened for their antimicrobial activities against<jats:italic> Escherichia coli <\/jats:italic>(clinical isolate)<jats:italic>, Klebsiella pneumoniae<\/jats:italic> (ATCC 13883),<jats:italic> Pseudomonas aeruginosa<\/jats:italic> (clinical isolate),<jats:italic> Pseudomonas aeruginosa<\/jats:italic> (ATCC 27853),<jats:italic> Staphylococcus aureus <\/jats:italic>(ATCC 25923)<jats:italic>, <\/jats:italic>MRSA (ATCC 33591),<jats:italic> Candida albicans<\/jats:italic> (clinical isolate), and<jats:italic> Candida parapsilosis<\/jats:italic> (ATCC 90018) using tetrazolium microtiter plate bioassay method.<jats:italic> Results<\/jats:italic>. Of the 35 tested mushroom extracts, extracts of three (3) mushrooms, namely,<jats:italic> Trametes <\/jats:italic>spp. (Arabuko-Sokoke forest),<jats:italic> Trametes<\/jats:italic>, and<jats:italic> Microporus<\/jats:italic> spp. (Kakamega forest), have shown promising antimicrobial activities against the tested organisms. The<jats:italic> S. aureus<\/jats:italic> (ATCC 25923),<jats:italic> P. aeruginosa<\/jats:italic> (ATCC 27853), and Methicillin-Resistant<jats:italic> Staphylococcus aureus<\/jats:italic> (MRSA) (ATCC 33591) were the most susceptible to chloroform extract of<jats:italic> Trametes<\/jats:italic> spp. collected from Arabuko-Sokoke forest. Of the tested organisms,<jats:italic> S. aureus<\/jats:italic> (ATCC 25923) was the most susceptible whereas<jats:italic> E. coli<\/jats:italic> was the most resistant organism to the hot water extract of<jats:italic> Trametes<\/jats:italic> spp. collected from Arabuko-Sokoke forest. Chloroform extract of<jats:italic> Microporus <\/jats:italic>spp. has shown the highest antibacterial activity against<jats:italic> S. aureus<\/jats:italic> (ATCC 25923), MRSA (ATCC 33591), and<jats:italic> K. pneumoniae<\/jats:italic> (ATCC 13883) but limited activity against<jats:italic> E.coli<\/jats:italic>. All extracts of the three wild mushrooms have shown the most antibacterial activities against<jats:italic> S. aureus <\/jats:italic>(ATCC 25923).<jats:italic> Conclusion<\/jats:italic>. The present study has shown that the extracts of the three wild mushrooms have shown promising antimicrobial activities against the tested organisms.<\/jats:p>","DOI":"10.1155\/2019\/6212673","type":"journal-article","created":{"date-parts":[[2019,2,18]],"date-time":"2019-02-18T18:33:51Z","timestamp":1550514831000},"page":"1-7","source":"Crossref","is-referenced-by-count":57,"title":["Determination of Antimicrobial Activity of Extracts of Indigenous Wild Mushrooms against Pathogenic Organisms"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2697-4818","authenticated-orcid":true,"given":"Gebreselema","family":"Gebreyohannes","sequence":"first","affiliation":[{"name":"Department of Biological and Chemical Engineering, Mekelle Institute of Technology, Mekelle University, Ethiopia"},{"name":"Molecular Biology and Biotechnology, Pan African University, Institute for Basic Sciences, Technology, and Innovation; Jomo Kenyatta University of Agriculture and 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