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Bitter taste receptors (T2Rs) mediate the recognition of bitter compounds belonging to a family of proteins known as G-protein coupled receptors. The aim of this study was to identify and evaluate the expression of<jats:italic>T2R7<\/jats:italic>in chicken tongue tissue and construct<jats:italic>cT2R7-1<\/jats:italic>and<jats:italic>cT2R7-2-<\/jats:italic>expressing HEK-293T cells to access the expression of<jats:italic>PLC\u03b22<\/jats:italic>and<jats:italic>ITPR3<\/jats:italic>after exposure with different concentrations of the bitter compounds. Using real-time PCR, we show that the relative expression level of<jats:italic>T2R7<\/jats:italic>mRNA in 5, 1, 0.1, and 10<jats:sup>\u22123<\/jats:sup>\u2009mM of camphor and erythromycin solutions and 5\u2009mM of chlorpheniramine maleate solutions was significantly higher than that in 50\u2009mM KCL solutions. We confirmed that the bitter taste receptor T2R7 and downstream signaling effectors are sensitive to different concentrations of bitter compounds. Moreover, T2R7-1 (corresponding to the unique haplotype of the Tibetan chicken) had higher sensitivity to bitter compounds compared with that of T2R7-2 (corresponding to the unique haplotype of the Jiuyuan black-chicken). These results provide great significance of taste response on dietary intake to improve chicken feeding efficiency in poultry production and have certain reference value for future taste research in other bird species.<\/jats:p>","DOI":"10.1155\/2019\/6301915","type":"journal-article","created":{"date-parts":[[2019,10,31]],"date-time":"2019-10-31T23:33:52Z","timestamp":1572564832000},"page":"1-12","source":"Crossref","is-referenced-by-count":4,"title":["Effect of Bitter Compounds on the Expression of Bitter Taste Receptor T2R7 Downstream Signaling Effectors in<i>cT2R7<\/i>\/pDisplay-G<i>\u03b1<\/i>16\/gust44\/pcDNA3.1 (+) Cells"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1429-7792","authenticated-orcid":true,"given":"Yuan","family":"Su","sequence":"first","affiliation":[{"name":"Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, 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