{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:32:17Z","timestamp":1792787537440,"version":"4.5.0"},"reference-count":41,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,1,20]],"date-time":"2019-01-20T00:00:00Z","timestamp":1547942400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["2008-03140"],"award-info":[{"award-number":["2008-03140"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["38\/128"],"award-info":[{"award-number":["38\/128"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Kingdom of Saudi Arabia","award":["2008-03140"],"award-info":[{"award-number":["2008-03140"]}]},{"name":"Kingdom of Saudi Arabia","award":["38\/128"],"award-info":[{"award-number":["38\/128"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2019,1,20]]},"abstract":"<jats:p>\n                    Fungal aetiology of keratitis\/corneal ulcer is considered to be one of the leading causes of ocular morbidity, particularly in developing countries including India. More importantly,\n                    <jats:italic>Fusarium<\/jats:italic>\n                    and\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    are reported commonly implicating corneal ulcer and against this background the present work was undertaken so as to understand the current epidemiological trend of the two fungal keratitis. During the project period, a total of 500 corneal scrapings were collected from suspected mycotic keratitis patients, of which 411 (82.2%) were culture positive for bacteria, fungi, and parasites. Among fungal aetiologies,\n                    <jats:italic>Fusarium<\/jats:italic>\n                    (216, 52.5% of 411) and\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    (68, 16.5% of 411) were predominantly determined. While the study revealed a male preponderance with both the fungal keratitis , it further brought out that polyene compounds (natamycin and amphotericin B) and azoles were active, respectively, against\n                    <jats:italic>Fusarium<\/jats:italic>\n                    spp. and\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    spp. Additionally, 94.1% of culture proven\n                    <jats:italic>Fusarium<\/jats:italic>\n                    keratitis and, respectively, 100% and 63.6% of\n                    <jats:italic>A. flavus<\/jats:italic>\n                    and\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    were confirmed by multiplex PCR. The sensitivity of the PCR employed in the present study was noted to be 10 fg\/\n                    <jats:italic>\u03bc<\/jats:italic>\n                    l, 1\u2009pg\/\n                    <jats:italic>\u03bc<\/jats:italic>\n                    l, and 300\u2009pg\/\n                    <jats:italic>\u03bc<\/jats:italic>\n                    l of DNA, respectively, for\n                    <jats:italic>Fusarium<\/jats:italic>\n                    ,\n                    <jats:italic>A. flavus,<\/jats:italic>\n                    and\n                    <jats:italic>A. fumigatus.<\/jats:italic>\n                    Alarming fact was that\n                    <jats:italic>Fusarium<\/jats:italic>\n                    and\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    regionally remained to be the common cause of mycotic keratitis and the\n                    <jats:italic>Fusarium<\/jats:italic>\n                    isolates had a higher antifungal resistance than\n                    <jats:italic>Aspergillus<\/jats:italic>\n                    strains against most of the test drugs.\n                  <\/jats:p>","DOI":"10.1155\/2019\/6395840","type":"journal-article","created":{"date-parts":[[2019,1,20]],"date-time":"2019-01-20T18:31:43Z","timestamp":1548009103000},"page":"1-9","source":"Crossref","is-referenced-by-count":120,"title":["Fungal Keratitis: Epidemiology, Rapid Detection, and Antifungal Susceptibilities of\n                    <i>Fusarium<\/i>\n                    and\n                    <i>Aspergillus<\/i>\n                    Isolates from Corneal Scrapings"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9636-4006","authenticated-orcid":true,"given":"Palanisamy","family":"Manikandan","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah, Saudi Arabia"},{"name":"Aravind Eye Hospital and Postgraduate Institute of Ophthalmology, Coimbatore, India"},{"name":"Greenlink Analytical and Research Laboratory India Private Limited, Coimbatore, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1933-8197","authenticated-orcid":true,"given":"Ahmed","family":"Abdel-hadi","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Sciences, College of Applied Medical Sciences, Majmaah University, Al Majmaah, Saudi Arabia"},{"name":"Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Assiut Branch, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yendrembam","family":"Randhir Babu Singh","sequence":"additional","affiliation":[{"name":"Microbial Resources Division, Institute of Bioresources and Sustainable Development, Takyel, Imphal, Manipur, 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