{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,15]],"date-time":"2026-07-15T15:24:19Z","timestamp":1784129059424,"version":"3.55.0"},"reference-count":65,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,1,14]],"date-time":"2019-01-14T00:00:00Z","timestamp":1547424000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["GB14-37368G"],"award-info":[{"award-number":["GB14-37368G"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura Cesk\u00e9 Republiky","doi-asserted-by":"publisher","award":["MUNI\/A\/1008\/2017"],"award-info":[{"award-number":["MUNI\/A\/1008\/2017"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010653","name":"Masaryk University","doi-asserted-by":"crossref","award":["GB14-37368G"],"award-info":[{"award-number":["GB14-37368G"]}],"id":[{"id":"10.13039\/501100010653","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100010653","name":"Masaryk University","doi-asserted-by":"crossref","award":["MUNI\/A\/1008\/2017"],"award-info":[{"award-number":["MUNI\/A\/1008\/2017"]}],"id":[{"id":"10.13039\/501100010653","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Dentistry"],"published-print":{"date-parts":[[2019,1,14]]},"abstract":"<jats:p>Recent studies have suggested a bidirectional relationship between chronic periodontitis (CP) and diabetes mellitus (DM). Immunoregulatory factors such as cytokines play an important role in etiopathogenesis of both diseases. The aim of this study was to analyze variability in interleukin-1 (<jats:italic>IL-1<\/jats:italic>) gene cluster and IL-1<jats:italic>\u03b2<\/jats:italic>plasma levels in patients with CP, DM, and a combination of both diseases. A total of 1016 individuals participating in this case-control study\u2014225 healthy controls, 264 patients with CP, 132 with type 1 diabetes (T1DM), and 395 patients with type 2 diabetes (T2DM)\u2014were genotyped using methods based on polymerase chain reaction for<jats:italic>IL-1<\/jats:italic>gene polymorphisms (<jats:italic>IL-1A<\/jats:italic>(\u2212889C\/T, rs1800587),<jats:italic>IL-1B<\/jats:italic>(+3953C\/T, rs1143634), and<jats:italic>IL-1RN<\/jats:italic>(gene for IL-1 receptor antagonist, IL-1RA, 86\u2009bp tandem repeats in intron 2)). Levels of IL-1<jats:italic>\u03b2<\/jats:italic>were measured by Luminex methods in subgroups of controls, CP, T1DM\u2009+\u2009CP, and T2DM\u2009+\u2009CP subjects. Although no significant associations were found in the genotype and allele frequencies of<jats:italic>IL-1A<\/jats:italic>(\u2212889C\/T), significant differences in the allele frequencies of<jats:italic>IL-1B<\/jats:italic>(+3953C\/T) were observed between controls and CP patients (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:mrow><\/mml:math>). In T1DM patients,<jats:italic>IL-1RN<\/jats:italic><mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:msup><mml:mrow\/><mml:mrow><mml:mi>\u2217<\/mml:mi><\/mml:mrow><\/mml:msup><\/mml:math>S<jats:italic>\u201c<\/jats:italic>short\u201d allele and<jats:italic>IL-1RN<\/jats:italic>12 genotype were significantly less frequent than those in controls (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:mrow><\/mml:math>). In haplotype analysis, TTL haplotype decreased the risk of CP development (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:mrow><\/mml:math>), whereas CCS and CTL haplotypes (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.01<\/mml:mn><\/mml:mrow><\/mml:math>and<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:mrow><\/mml:math>) were associated with T1DM. Although IL-1<jats:italic>\u03b2<\/jats:italic>levels were measured significantly higher in mononuclear cells after stimulation by mitogens, HSP70, or selected periodontal bacteria than in unstimulated cells,<jats:italic>IL-1<\/jats:italic>genotypes did not correlate with circulating IL-1<jats:italic>\u03b2<\/jats:italic>levels. In the Czech population, significant associations between the<jats:italic>IL-1B<\/jats:italic>polymorphism with CP and the<jats:italic>IL-1RN<\/jats:italic>variant with T1DM were found. Haplotype analysis suggests that variability in<jats:italic>IL-1<\/jats:italic>gene cluster may be one of the factors in the CP and T1DM pathogenesis, although single variants of these polymorphisms are not substantial for protein production.<\/jats:p>","DOI":"10.1155\/2019\/6802349","type":"journal-article","created":{"date-parts":[[2019,1,14]],"date-time":"2019-01-14T19:17:51Z","timestamp":1547493471000},"page":"1-10","source":"Crossref","is-referenced-by-count":11,"title":["Interleukin-1 Gene Variability and Plasma Levels in Czech Patients with Chronic Periodontitis and Diabetes Mellitus"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0953-3615","authenticated-orcid":true,"given":"Petra","family":"Borilova Linhartova","sequence":"first","affiliation":[{"name":"Clinic of Stomatology, Institutions Shared with St. Anne\u2019s Faculty Hospital, Faculty of Medicine, Masaryk University, Brno 60200, Czech Republic"},{"name":"Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno 62500, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0727-0180","authenticated-orcid":true,"given":"Hana","family":"Poskerova","sequence":"additional","affiliation":[{"name":"Clinic of Stomatology, Institutions Shared with St. Anne\u2019s Faculty Hospital, Faculty of Medicine, Masaryk University, Brno 60200, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Tomandlova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Faculty of Medicine, Masaryk University, Brno 62500, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5277-6474","authenticated-orcid":true,"given":"Jirina","family":"Bartova","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Experimental Dental Medicine, General University Hospital and First Faculty of Medicine, Charles University, Prague 12000, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9548-0630","authenticated-orcid":true,"given":"Katerina","family":"Kankova","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno 62500, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonin","family":"Fassmann","sequence":"additional","affiliation":[{"name":"Clinic of Stomatology, Institutions Shared with St. Anne\u2019s Faculty Hospital, Faculty of Medicine, Masaryk University, Brno 60200, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7610-8929","authenticated-orcid":true,"given":"Lydie","family":"Izakovicova Holla","sequence":"additional","affiliation":[{"name":"Clinic of Stomatology, Institutions Shared with St. Anne\u2019s Faculty Hospital, Faculty of Medicine, Masaryk University, Brno 60200, Czech Republic"},{"name":"Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno 62500, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051x.1998.tb02419.x"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051x.2005.00821.x"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1902\/annals.2001.6.1.125"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2011.106"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2009.08.006"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1111\/prd.12089"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/137919"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a007724"},{"issue":"2","key":"9","first-page":"107","volume":"7","year":"2009","journal-title":"Oral Health and Preventive Dentistry"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.14219\/jada.archive.2006.0404"},{"key":"11","doi-asserted-by":"crossref","first-page":"1627","DOI":"10.1182\/blood.V77.8.1627.1627","volume":"77","year":"1991","journal-title":"Blood"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/0888-7543(92)90137-h"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2003.74.8.1183"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2008.070203"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2009.090134"},{"issue":"3","key":"16","doi-asserted-by":"crossref","first-page":"375","DOI":"10.4103\/0970-9290.138343","volume":"25","year":"2014","journal-title":"Indian Journal of Dental Research"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-015-0132-5"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.3109\/13813455.2010.551135"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/342351"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2979846"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780380210"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051x.1997.tb01187.x"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1007\/bf00217368"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2013.06.026"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/185757"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.2217\/14622416.7.5.683"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-011-2342-y"},{"issue":"2","key":"28","first-page":"63","volume":"27","year":"2014","journal-title":"American Journal of Dentistry"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1177\/154405910508401211"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051x.2007.01132.x"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2003.300106.x"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2012.792"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-051x.2000.027009682.x"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1177\/00220345010800080301"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051x.2006.00983.x"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2007.060135"},{"issue":"2","key":"37","first-page":"48","volume":"104","year":"2004","journal-title":"\u010cesk\u00e1 stomatologie"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2018.05.067"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.4317\/medoral.21233"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2012.01.016"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1002\/gepi.10200"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1590\/s1806-83242012000700006"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.3031"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1080\/08916930802292510"},{"issue":"19","key":"45","first-page":"3701","volume":"19","year":"2015","journal-title":"European Review for Medical and Pharmacological Sciences"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.1998.00300.x"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1995.tb05549.x"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2004.02531.x"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1007\/s00431-002-1030-9"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2010.02294.x"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2009-0666"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2006.09.012"},{"issue":"7","key":"53","doi-asserted-by":"crossref","first-page":"259","DOI":"10.4103\/0019-5359.42021","volume":"62","year":"2008","journal-title":"Indian Journal of Medical Sciences"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302x.2006.00292.x"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051x.2007.01057.x"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2012.01500.x"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2012.110616"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1902\/annals.1998.3.1.40"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2010.01324.x"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2014.02.002"},{"issue":"4","key":"61","first-page":"413","volume":"132","year":"2004","journal-title":"Revista Me\u0301dica de Chile"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0142976"},{"issue":"4","key":"63","first-page":"443","volume":"44","year":"2014","journal-title":"Annals of Clinical and Laboratory Science"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.1016\/j.orcp.2015.01.005"},{"key":"65","doi-asserted-by":"publisher","DOI":"10.1902\/jop.2013.130281"}],"container-title":["International Journal of Dentistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijd\/2019\/6802349.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijd\/2019\/6802349.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ijd\/2019\/6802349.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,11,14]],"date-time":"2019-11-14T05:46:14Z","timestamp":1573710374000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ijd\/2019\/6802349\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,14]]},"references-count":65,"alternative-id":["6802349","6802349"],"URL":"https:\/\/doi.org\/10.1155\/2019\/6802349","relation":{},"ISSN":["1687-8728","1687-8736"],"issn-type":[{"value":"1687-8728","type":"print"},{"value":"1687-8736","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,14]]}}}