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However, transduction and transgene expression of rAAVs can differ across<jats:italic>in vitro<\/jats:italic>and ex vivo cellular transduction strategies. This study compared 11 rAAV serotypes, carrying one reporter transgene cassette containing a cytomegalovirus immediate-early enhancer (eCMV) and chicken beta actin (CBA) promoter driving the expression of an enhanced green-fluorescent protein (eGFP) gene, which was transduced into four different cell types: human iPSC, iPSC-derived RPE, iPSC-derived cortical, and dissociated embryonic day 18 rat cortical neurons. Each cell type was exposed to three multiplicity of infections (MOI: 1E4, 1E5, and 1E6\u2009vg\/cell). After 24, 48, 72, and 96\u2009h posttransduction, GFP-expressing cells were examined and compared across dosage, time, and cell type. Retinal pigmented epithelium showed highest AAV-eGFP expression and iPSC cortical the lowest. At an MOI of 1E6\u2009vg\/cell, all serotypes show measurable levels of AAV-eGFP expression; moreover, AAV7m8 and AAV6 perform best across MOI and cell type. We conclude that serotype tropism is not only capsid dependent but also cell type plays a significant role in transgene expression dynamics.<\/jats:p>","DOI":"10.1155\/2019\/7281912","type":"journal-article","created":{"date-parts":[[2019,1,17]],"date-time":"2019-01-17T23:32:26Z","timestamp":1547767946000},"page":"1-11","source":"Crossref","is-referenced-by-count":29,"title":["Comparative AAV-eGFP Transgene Expression Using Vector Serotypes 1\u20139, 7m8, and 8b in Human Pluripotent Stem Cells, RPEs, and Human and Rat Cortical Neurons"],"prefix":"10.1155","volume":"2019","author":[{"given":"Thu T.","family":"Duong","sequence":"first","affiliation":[{"name":"F.M. 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