{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:18:40Z","timestamp":1792721920186,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,5,16]],"date-time":"2019-05-16T00:00:00Z","timestamp":1557964800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772112"],"award-info":[{"award-number":["81772112"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571937"],"award-info":[{"award-number":["81571937"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81772112"],"award-info":[{"award-number":["81772112"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571937"],"award-info":[{"award-number":["81571937"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2019,5,16]]},"abstract":"<jats:p>Apoptosis of CD4<jats:sup>+<\/jats:sup> T cells plays a central role in the progression of sepsis because it is associated with subsequent immunosuppression and the lack of specific treatment. Thus, developing therapeutic strategies to attenuate the apoptosis of CD4<jats:sup>+<\/jats:sup> T cells in sepsis is critical. Several studies have demonstrated that Mdivi-1, which is a selective inhibitor of the dynamin-related protein 1 (Drp1), attenuates apoptosis of myocardial cells and neurons during various pathologic states. The present study revealed the impact of Mdivi-1 on the apoptosis of CD4<jats:sup>+<\/jats:sup> T cells in sepsis and the potential underlying mechanisms. We used lipopolysaccharide (LPS) stimulation and cecal ligation and puncture (CLP) surgery as sepsis models <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>, respectively. Our results showed that Mdivi-1 attenuated the apoptosis of CD4<jats:sup>+<\/jats:sup> T cells both <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>. The potential mechanism underlying the protective effect of Mdivi-1 involved Mdivi-1 reestablishing mitochondrial fusion-fission balance in sepsis, as reflected by the expression of the mitofusin 2 (MFN2) and optic atrophy 1 (OPA1) , Drp1 translocation, and mitochondrial morphology, as observed by electron microscopy. Moreover, Mdivi-1 treatment reduced reactive oxygen species (ROS) production and prevented the induction of endoplasmic reticulum stress (ERS) and associated apoptosis<jats:italic>.<\/jats:italic> After using tunicamycin to activate ER stress, the protective effect of Mdivi-1 on CD4<jats:sup>+<\/jats:sup> T cells was reversed. Our results suggested that Mdivi-1 ameliorated apoptosis in CD4<jats:sup>+<\/jats:sup> T cells by reestablishing mitochondrial fusion-fission balance and preventing the induction of endoplasmic reticulum stress in experimental sepsis.<\/jats:p>","DOI":"10.1155\/2019\/7329131","type":"journal-article","created":{"date-parts":[[2019,5,16]],"date-time":"2019-05-16T19:33:45Z","timestamp":1558035225000},"page":"1-14","source":"Crossref","is-referenced-by-count":23,"title":["Mdivi-1 Protects CD4<sup>+<\/sup> T Cells against Apoptosis via Balancing Mitochondrial Fusion-Fission and Preventing the Induction of Endoplasmic Reticulum Stress in Sepsis"],"prefix":"10.1155","volume":"2019","author":[{"given":"You","family":"Wu","sequence":"first","affiliation":[{"name":"Emergency Department, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China"},{"name":"Wenzhou Municipal Key Laboratory of Emergency, Critical Care and Disaster Medicine, The First Affiliated Hospital of Wenzhou 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