{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:50:40Z","timestamp":1787017840152,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,6,2]],"date-time":"2019-06-02T00:00:00Z","timestamp":1559433600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Parasitology Research"],"published-print":{"date-parts":[[2019,6,2]]},"abstract":"<jats:p>\n                    The pathological agents\n                    <jats:italic>Toxoplasma gondii, Ancylostoma caninum,<\/jats:italic>\n                    and\n                    <jats:italic>Toxocara canis<\/jats:italic>\n                    are widely distributed zoonotic parasites with high prevalence in tropical and subtropical regions of the world. The aim of the present study was to determine the presence of DNA from these parasites in sand samples from the sand playgrounds in the southeastern region of Mexico. Samples of sand were collected from 68 playgrounds in public parks in the city of Merida, Yucatan, which is the main urban area in the southeast of Mexico. The samples were examined using nested PCR to detect the SAG1 gene from\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    , and endpoint PCR for the amplification of ITS-2 and rRNA-ITS2 genes from\n                    <jats:italic>Toxocara canis<\/jats:italic>\n                    and\n                    <jats:italic>Ancylostoma caninum,<\/jats:italic>\n                    respectively. The presence of\n                    <jats:italic>T. gondii<\/jats:italic>\n                    DNA was detected in 11.8% (8\/68) samples, DNA from\n                    <jats:italic>A. caninum<\/jats:italic>\n                    and\n                    <jats:italic>T. canis<\/jats:italic>\n                    was not detected. Results indicate that playgrounds from the studied sandboxes are contaminated with\n                    <jats:italic>T. gondii<\/jats:italic>\n                    oocysts and may represent a risk of infection for people in contact with the sand, especially for preschoolers.\n                  <\/jats:p>","DOI":"10.1155\/2019\/7409076","type":"journal-article","created":{"date-parts":[[2019,6,2]],"date-time":"2019-06-02T19:35:06Z","timestamp":1559504106000},"page":"1-6","source":"Crossref","is-referenced-by-count":7,"title":["Screening of Zoonotic Parasites in Playground Sandboxes of Public Parks from Subtropical Mexico"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6359-131X","authenticated-orcid":true,"given":"Gonzalo A.","family":"Pacheco-Ortega","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Celular, Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi\u201d, Universidad Aut\u00f3noma de Yucat\u00e1n, 97000 M\u00e9rida, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8128-9384","authenticated-orcid":true,"given":"Jos\u00e9 I.","family":"Chan-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Celular, Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi\u201d, Universidad Aut\u00f3noma de Yucat\u00e1n, 97000 M\u00e9rida, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8502-8023","authenticated-orcid":true,"given":"Antonio","family":"Ortega-Pacheco","sequence":"additional","affiliation":[{"name":"Depto. de Medicina Interna y Cirug\u00eda. Campus de Ciencias Biol\u00f3gicas y Agropecuarias, Universidad Aut\u00f3noma de Yucat\u00e1n, 97100 M\u00e9rida, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3407-9314","authenticated-orcid":true,"given":"Eugenia","family":"Guzm\u00e1n-Mar\u00edn","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Celular, Centro de Investigaciones Regionales \u201cDr. Hideyo Noguchi\u201d, Universidad Aut\u00f3noma de Yucat\u00e1n, 97000 M\u00e9rida, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa","family":"Edwards","sequence":"additional","affiliation":[{"name":"Office for Undergraduate Research and Artistry, The Institute for Learning and Teaching, Colorado State University, Fort Collins, CO 80521, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark A.","family":"Brown","sequence":"additional","affiliation":[{"name":"Office for Undergraduate Research and 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