{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T23:41:32Z","timestamp":1779147692268,"version":"3.51.4"},"reference-count":24,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T00:00:00Z","timestamp":1552953600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002383","name":"King Saud University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002383","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Analytical Methods in Chemistry"],"published-print":{"date-parts":[[2019,3,19]]},"abstract":"<jats:p><jats:italic>Plicosepalus<\/jats:italic> is an important genus of the Loranthaceae family, and it is a semiparasitic plant grown in Saudi Arabia<jats:italic>,<\/jats:italic> traditionally used as a cure for diabetes and cancer in human and for increasing lactation in cattle. A flavonoid quercetin (<jats:bold>P1<\/jats:bold>), (-)-catechin (<jats:bold>P2<\/jats:bold>), and a flavane gallate 2S,3R-3,3\u2032,4\u2032,5,7-pentahydroxyflavane-5-O-gallate (<jats:bold>P3<\/jats:bold>) were isolated from the methanol extract of the aerial parts of <jats:italic>P. curviflorus<\/jats:italic> (PCME). The PCME and the isolated compounds were subjected to pharmacological assays to estimate peroxisome proliferator-activated receptors PPAR<jats:italic>\u03b1<\/jats:italic> and PPAR<jats:italic>\u03b3<\/jats:italic> agonistic, anti-inflammatory, cytotoxic, and antimicrobial activities. Results proved for the first time the dual PPAR activation effect of the PCME and catechin (<jats:bold>P2<\/jats:bold>), in addition to the promising anti-inflammatory activity of the flavonoid quercetin (<jats:bold>P1<\/jats:bold>). Interestingly, both PCME and isolated compounds showed potent antioxidant activities while no antimicrobial effect against certain microbial strains had been reported from the extract and the isolated compounds. Based on the pharmacological importance of these compounds, an HPTLC validated method was developed for the simultaneous estimation of these compounds in PCME. It was found to furnish a compact and sharp band of compounds <jats:bold>P1<\/jats:bold>, <jats:bold>P2<\/jats:bold>, and <jats:bold>P3<\/jats:bold> at <jats:italic>R<\/jats:italic><jats:sub>f<\/jats:sub>\u2009=\u20090.34, 0.47, and 0.65, respectively, using dichloromethane, methanol, and formic acid (90\u2009:\u20099.5\u2009:\u20090.5, (v\/v\/v)) as the mobile phase. Compounds <jats:bold>P1<\/jats:bold>, <jats:bold>P2<\/jats:bold>, and <jats:bold>P3<\/jats:bold> were found to be 11.06, 10.9, 6.96\u2009<jats:italic>\u03bc<\/jats:italic>g\/mg, respectively, in PCME. The proposed HPTLC method offers a sensitive, precise, and specific analytical tool for the quantification of quercetin, catechin, and flavane gallates in <jats:italic>P. curviflorus<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2019\/7435909","type":"journal-article","created":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T19:40:01Z","timestamp":1553024401000},"page":"1-8","source":"Crossref","is-referenced-by-count":13,"title":["Pharmacological Evaluation of Secondary Metabolites and Their Simultaneous Determination in the Arabian Medicinal Plant <i>Plicosepalus curviflorus<\/i> Using HPTLC Validated Method"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9421-4127","authenticated-orcid":true,"given":"Raha","family":"Orfali","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3318-9861","authenticated-orcid":true,"given":"Shagufta","family":"Perveen","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nasir Ali","family":"Siddiqui","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Perwez","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tawfeq Abdullah","family":"Alhowiriny","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Areej Mohammad","family":"Al-Taweel","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, College of Pharmacy, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sami","family":"Al-Yahya","sequence":"additional","affiliation":[{"name":"National Center for Biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh 11461, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fuad","family":"Ameen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Science, King Saud University, P.O. Box 2457, Riyadh 11451, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Najwa","family":"Majrashi","sequence":"additional","affiliation":[{"name":"National Center for Biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh 11461, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khulud","family":"Alluhayb","sequence":"additional","affiliation":[{"name":"National Center for Biotechnology, Life Science and Environment Research Institute, King Abdulaziz City for Science and Technology (KACST), P.O. Box 6086, Riyadh 11461, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bandar","family":"Alghanem","sequence":"additional","affiliation":[{"name":"Medical Core Facility and Research Platforms, King Abdullah International Medical Research Center\/King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs, Riyadh 11426, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hayat","family":"Shaibah","sequence":"additional","affiliation":[{"name":"Medical Core Facility and Research Platforms, King Abdullah International Medical Research Center\/King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard Health Affairs, Riyadh 11426, Saudi Arabia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shabana Iqrar","family":"Khan","sequence":"additional","affiliation":[{"name":"National Center for Natural Products Research, School of Pharmacy, University of Mississippi, Oxford 38677, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-7847.194044"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.4314\/tjpr.v13i9.26"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.3390\/ijms12129342"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1186\/1749-8546-5-13"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21121679"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2007.01.018"},{"key":"7","volume":"236","year":"1996"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.5897\/ajb10.1570"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.3109\/13880208509069018"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1515\/znc-2015-0231"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2012.09.010"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1089\/acm.2014.5336.abstract"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsps.2016.05.004"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.3923\/ijp.2015.372.376"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1007\/bf02624436"},{"issue":"3","key":"16","first-page":"47","volume-title":"Screening methods for antibacterial and antiviral agents from higher plants","volume":"6","year":"1991"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.5560\/znc.2014-0047"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.12.3442"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2014.07.018"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0172273"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2005.09.002"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1616\/1476-2137.14184"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjp.2016.05.017"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.4103\/0974-8490.210326"}],"container-title":["Journal of Analytical Methods in Chemistry"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jamc\/2019\/7435909.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jamc\/2019\/7435909.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jamc\/2019\/7435909.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,3,19]],"date-time":"2019-03-19T19:40:03Z","timestamp":1553024403000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jamc\/2019\/7435909\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,3,19]]},"references-count":24,"alternative-id":["7435909","7435909"],"URL":"https:\/\/doi.org\/10.1155\/2019\/7435909","relation":{},"ISSN":["2090-8865","2090-8873"],"issn-type":[{"value":"2090-8865","type":"print"},{"value":"2090-8873","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,3,19]]}}}