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Carvedilol is a nonselective beta-blocker used to reduce portal hypertension. This study investigated the effects and potential mechanisms of carvedilol in angiotensin II- (Ang II-) induced hepatic stellate cell (HSC) proliferation and contraction. <jats:italic>Methods<\/jats:italic>. The effect of carvedilol on HSC proliferation was measured by Cell Counting Kit-8 (CCK-8). Cell cycle progression and apoptosis in HSCs were determined by flow cytometry. A collagen gel assay was used to confirm HSC contraction. The extent of liver fibrosis in mice was evaluated by hematoxylin-eosin (H&amp;E) and Sirius Red staining. Western blot analyses were performed to detect the expression of collagen I, collagen III, <jats:italic>\u03b1<\/jats:italic>-smooth muscle actin (<jats:italic>\u03b1<\/jats:italic>-SMA), Ang II type I receptor (AT1R), RhoA, Rho-kinase 2 (ROCK2), and others. <jats:italic>Results<\/jats:italic>. The results showed that carvedilol inhibited HSC proliferation and arrested the cell cycle at the G0\/G1 phase in a dose-dependent manner. Carvedilol also modulated Bcl-2 family proteins and increased apoptosis in Ang II-treated HSCs. Furthermore, carvedilol inhibited HSC contraction induced by Ang II, an effect that was associated with AT1R-mediated RhoA\/ROCK2 pathway interference. In addition, carvedilol reduced <jats:italic>\u03b1<\/jats:italic>-SMA expression and collagen deposition and attenuated liver fibrosis in carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>)-treated mice. The <jats:italic>in vivo<\/jats:italic> data further confirmed that carvedilol inhibited the expression of angiotensin-converting enzyme (ACE), AT1R, RhoA, and ROCK2. <jats:italic>Conclusions<\/jats:italic>. The results indicated that carvedilol dose-dependently inhibited Ang II-induced HSC proliferation by impeding cell cycle progression, thus alleviating hepatic fibrosis. Furthermore, carvedilol could inhibit Ang II-induced HSC contraction by interfering with the AT1R-mediated RhoA\/ROCK2 pathway.<\/jats:p>","DOI":"10.1155\/2019\/7932046","type":"journal-article","created":{"date-parts":[[2019,11,7]],"date-time":"2019-11-07T18:31:53Z","timestamp":1573151513000},"page":"1-15","source":"Crossref","is-referenced-by-count":19,"title":["Carvedilol Inhibits Angiotensin II-Induced Proliferation and Contraction in Hepatic Stellate Cells through the RhoA\/Rho-Kinase Pathway"],"prefix":"10.1155","volume":"2019","author":[{"given":"Ying","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, Shandong Province, China"},{"name":"Shandong Provincial Engineering and Technological Research Center for Liver Diseases Prevention and Control, Jinan, 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