{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T10:57:17Z","timestamp":1789383437877,"version":"build-2803163510"},"reference-count":58,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T00:00:00Z","timestamp":1564531200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"crossref","award":["2016YFD0500501"],"award-info":[{"award-number":["2016YFD0500501"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2019,7,31]]},"abstract":"<jats:p>Hydrogen sulfide (H<jats:sub>2<\/jats:sub>S) is one of the main pollutants in the atmosphere, which is a serious threat to human health. The decomposition of sulfur-containing organics in chicken houses could produce a large amount of H<jats:sub>2<\/jats:sub>S, thereby damaging poultry health. In this study, one-day-old broilers were selected and exposed to 4 or 20\u2009ppm of H<jats:sub>2<\/jats:sub>S gas (0-3 weeks: <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mn>4<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>0.5<\/mml:mn><mml:mtext>\u2009<\/mml:mtext><mml:mtext>ppm<\/mml:mtext><\/mml:math>, 4-6 weeks: <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mn>20<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>0.5<\/mml:mn><mml:mtext>\u2009<\/mml:mtext><mml:mtext>ppm<\/mml:mtext><\/mml:math>). The spleen samples were collected immediately after the chickens were euthanized at 2, 4, and 6 weeks. The histopathological and ultrastructural observations showed obvious necrosis characteristics of H<jats:sub>2<\/jats:sub>S-exposed spleens. H<jats:sub>2<\/jats:sub>S exposure suppressed GSH, CAT, T-AOC, and SOD activities; increased NO, H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>, and MDA content and iNOS activity; and induced oxidative stress. ATPase activities and the expressions of energy metabolism-related genes were significantly decreased. Also, the expressions of related necroptosis (RIPK1, RIPK3, MLKL, TAK1, TAB2, and TAB3) were significantly increased, and the MAPK pathway was activated. Besides, H<jats:sub>2<\/jats:sub>S exposure activated the NF-<jats:italic>\u03ba<\/jats:italic>B classical pathway and induced TNF-<jats:italic>\u03b1<\/jats:italic> and IL-1<jats:italic>\u03b2<\/jats:italic> release. Taken together, we conclude that H<jats:sub>2<\/jats:sub>S exposure induces oxidative stress and energy metabolism dysfunction; evokes necroptosis; activates the MAPK pathway, eventually triggering the NF-<jats:italic>\u03ba<\/jats:italic>B pathway; and promotes inflammatory response in chicken spleens.<\/jats:p>","DOI":"10.1155\/2019\/8061823","type":"journal-article","created":{"date-parts":[[2019,7,31]],"date-time":"2019-07-31T19:31:17Z","timestamp":1564601477000},"page":"1-13","source":"Crossref","is-referenced-by-count":61,"title":["Hydrogen Sulfide Gas Exposure Induces Necroptosis and Promotes Inflammation through the MAPK\/NF-<i>\u03ba<\/i>B Pathway in Broiler Spleen"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7381-4281","authenticated-orcid":true,"given":"Qianru","family":"Chi","sequence":"first","affiliation":[{"name":"College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, 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