{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T22:33:24Z","timestamp":1781217204367,"version":"3.54.1"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,1,16]],"date-time":"2019-01-16T00:00:00Z","timestamp":1547596800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"crossref","award":["2018YFC1002105"],"award-info":[{"award-number":["2018YFC1002105"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2019,1,16]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. The modified Bushen Antai recipe (BSAT) is a centuries-old traditional Chinese medicine that we use in our center as a therapy against pregnancy loss. Our study aimed to explore the potential benefit and mechanism of BSAT in pregnant rats with mifepristone-induced pregnancy loss.<jats:italic>Materials and Methods<\/jats:italic>. The signature compounds of the eight BSAT ingredients were analyzed by high-performance liquid chromatography (HPLC). The BSAT group (n = 8) was treated daily with 6.3 ml\/kg BSAT from gestation day (D) 0.5 to 10.5 and once with 1.25 mg\/kg mifepristone on D 10.5. Normal saline replaced BSAT in the model group (n = 8), and both BSAT and mifepristone in the control group (n = 8). Morphological and histological analyses were performed on D 13.5.<jats:italic>Results<\/jats:italic>. BSAT contains eight medicinal ingredients including<jats:italic>Cuscuta chinensis<\/jats:italic>and<jats:italic>Dipsacus asperoides<\/jats:italic>. The HPLC analysis detected the signature compounds of seven medicinal ingredients in the extract. Embryo resorption rate in the BSAT group was significantly lower than that in the model group, although the number of surviving embryos was similar between the two groups. Hematoxylin and eosin (HE) staining suggested that the maximum cross-sectional area of the placenta and the area ratio of the placental labyrinth in the BSAT group were higher than those in the model group. Immunohistochemical (IHC) staining indicated that the expression of ki67, estrogen receptor alpha (ER<jats:italic>\u03b1<\/jats:italic>), and progesterone receptor (PR) in the placental labyrinth of the BSAT group was higher than that of the model group. Furthermore, the protein levels of ER<jats:italic>\u03b1<\/jats:italic>, PR, phospho-Akt\/Akt, and phospho-Erk1\/2\/Erk1\/2 in the BSAT group were higher than those in the control group. The mRNA levels of ER<jats:italic>\u03b1<\/jats:italic>and PR in the BSAT group were higher than those in the control group.<jats:italic>Conclusions<\/jats:italic>. BSAT may induce estrogen and progesterone receptors by phosphorylation via the classic Akt and Erk1\/2 signaling pathways in the maternal-fetal interface of pregnant rats, thereby reducing the pregnancy loss rate and improving the live birth rate.<\/jats:p>","DOI":"10.1155\/2019\/8312020","type":"journal-article","created":{"date-parts":[[2019,1,16]],"date-time":"2019-01-16T23:32:19Z","timestamp":1547681539000},"page":"1-11","source":"Crossref","is-referenced-by-count":7,"title":["The Modified Bushen Antai Recipe Upregulates Estrogen and Progesterone Receptors at the Maternal-Fetal Interface in Pregnant Rats with Mifepristone-Induced Pregnancy Loss"],"prefix":"10.1155","volume":"2019","author":[{"given":"Li","family":"Sun","sequence":"first","affiliation":[{"name":"Reproductive Medicine Center, Obstetrics and Gynecology Department, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, China"},{"name":"Key Laboratory of Reproductive Dysfunction Diseases and Fertility Remodelling of Liaoning Province, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengwei","family":"Yuan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Health Ministry for Congenital Malformation, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingyan","family":"Jian","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shengjing Hospital of China Medical University, Shenyang 110022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghua","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Shengjing Hospital of China Medical University, Shenyang 110022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Obstetrics and Gynecology Department, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, China"},{"name":"Key Laboratory of Reproductive Dysfunction Diseases and Fertility Remodelling of Liaoning Province, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6401-1919","authenticated-orcid":true,"given":"Jichun","family":"Tan","sequence":"additional","affiliation":[{"name":"Reproductive Medicine Center, Obstetrics and Gynecology Department, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, China"},{"name":"Key Laboratory of Reproductive Dysfunction Diseases and Fertility Remodelling of Liaoning Province, Shengjing Hospital Affiliated to China Medical University, Shenyang 110022, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-0528.2009.02427.x"},{"key":"2","year":"2012"},{"issue":"6","key":"3","first-page":"827","volume":"70","year":"1987","journal-title":"Obstetrics & Gynecology"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1002\/uog.204"},{"issue":"21","key":"5","doi-asserted-by":"crossref","first-page":"4719","DOI":"10.1242\/dev.127.21.4719","volume":"127","year":"2000","journal-title":"Development"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2012.01.003"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-017-1108-y"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2018.07.020"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2015.09.041"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2016.12126"},{"issue":"12","key":"11","first-page":"1074","volume":"48","year":"2007","journal-title":"Singapore Medical Journal"},{"issue":"2, article cd003576","key":"12","year":"2005","journal-title":"Cochrane Database of Systematic Reviews"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.3109\/09513590.2015.1121982"},{"key":"14","volume":"3, article cd005943","year":"2011","journal-title":"Cochrane Database of Systematic Reviews"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD007422.pub2"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD008510.pub2"},{"issue":"6","key":"17","first-page":"28","volume":"18","year":"2012","journal-title":"Alternative Therapies In Health And Medicine"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/3852196"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0207580"},{"issue":"8","key":"20","first-page":"1251","volume":"34","year":"2011","journal-title":"Journal of Chinese medicinal materials"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00437"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.02.025"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/8656740"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/3709874"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.6205"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.10.015"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-13-141"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.02.019"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.contraception.2012.10.027"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.3109\/09513590.2013.856410"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.18632\/aging.100721"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1093\/humrep\/dep099"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2015.2548"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/j.contraception.2016.03.009"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejogrb.2011.09.006"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.104141"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1053\/plac.2001.0738"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/j.placenta.2015.12.019"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.6061\/clinics\/2017(05)02"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1016\/j.rbmo.2013.06.010"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1126\/science.1197454"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0705749104"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-21-2"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1093\/humupd\/dmu056"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1210\/er.2014-1045"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075282"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gax015"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/8.5.465"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12473"},{"issue":"2","key":"50","first-page":"104","volume":"6","year":"2014","journal-title":"American Journal of Translational Research"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2019\/8312020.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2019\/8312020.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2019\/8312020.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,10]],"date-time":"2022-09-10T12:11:06Z","timestamp":1662811866000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/ecam\/2019\/8312020\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,1,16]]},"references-count":50,"alternative-id":["8312020","8312020"],"URL":"https:\/\/doi.org\/10.1155\/2019\/8312020","relation":{},"ISSN":["1741-427X","1741-4288"],"issn-type":[{"value":"1741-427X","type":"print"},{"value":"1741-4288","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,1,16]]}}}