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Therefore, in this study, a new type of recombinant adenovirus expressing the spike (S) protein of porcine epidemic diarrhea virus (PEDV), rAd-PEDV-S, was generated, and its characteristics were determined. Then, its efficacy as a vaccine candidate was evaluated in 4-week-old pigs. The results showed that the S protein could be well expressed at a high level in rAd-PEDV-S-infected cells and that the viral titers could reach 10<jats:sup>11<\/jats:sup>\u2009PFU\/mL. Further animal experimental results showed that rAd-PEDV-S elicited a significant PEDV-specific humoral immune response after vaccination (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:mrow><\/mml:math>). In addition, rAd-PEDV-S provided partial protection for pigs against the highly virulent PEDV challenge. The results presented in this study indicate that the adenovirus vector can be used as a vaccine delivery vector for the development of a PEDV vaccine and is a promising novel vaccine candidate for future prevention and control of porcine epidemic diarrhea (PED), but its efficacy still needs to be improved in the future.<\/jats:p>","DOI":"10.1155\/2019\/8530273","type":"journal-article","created":{"date-parts":[[2019,10,8]],"date-time":"2019-10-08T19:30:46Z","timestamp":1570563046000},"page":"1-8","source":"Crossref","is-referenced-by-count":13,"title":["Evaluation of the Efficacy of a Recombinant Adenovirus Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus in Pigs"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1960-0252","authenticated-orcid":true,"given":"Xinsheng","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal Virology of Ministry of 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