{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,6]],"date-time":"2025-12-06T21:47:37Z","timestamp":1765057657435,"version":"3.37.3"},"reference-count":53,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T00:00:00Z","timestamp":1559606400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conseil R\u00e9gional Poitou-Charentes, Universit\u00e9 de Poitiers"},{"name":"CHU de Poitiers"},{"name":"Fondation Transplantation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2019,6,4]]},"abstract":"<jats:p>Ischemia-reperfusion (IR) injury is unavoidable during organ transplantation and impacts graft quality. New paradigms are emerging including preservation at higher temperature than \u201chypothermia\u201d or \u201ccold\u201d: although 4\u00b0C remains largely used for kidney preservation, recent studies challenged this choice. We and others hypothesized that a higher preservation temperature, closer to physiological regimen, could improve organ quality. For this purpose, we used an<jats:italic> in vitro<\/jats:italic> model of endothelial cells exposed to hypoxia-reoxygenation sequence (mimicking IR) and an<jats:italic> ex vivo<\/jats:italic> ischemic pig kidneys static storage model.<jats:italic> In vitro<\/jats:italic>, 19\u00b0C, 27\u00b0C, and 32\u00b0C provided protection against injuries versus 4\u00b0C, by reducing cell death, mitochondrial dysfunction, leukocyte adhesion, and inflammation. However,<jats:italic> ex vivo<\/jats:italic>, the benefits of 19\u00b0C or 32\u00b0C were limited, showing similar levels of tissue preservation damage.<jats:italic> Ex vivo<\/jats:italic> 4\u00b0C-preserved kidneys displayed a trend towards reduced damage, including apoptosis. Macrophage infiltration, tubulitis, and necrosis were increased in the 19\u00b0C and 32\u00b0C versus 4\u00b0C preserved kidneys. Thus, despite a trend for an advantage of subnormothermia as preservation temperature, our<jats:italic> in vitro<\/jats:italic> and<jats:italic> ex vivo<\/jats:italic> models bring different insights in terms of preservation temperature effect. This study suggests that temperature optimization for kidney preservation will require thorough investigation, combining the use of complementary relevant models and the design of elaborated preservation solution and new technologies.<\/jats:p>","DOI":"10.1155\/2019\/8572138","type":"journal-article","created":{"date-parts":[[2019,6,4]],"date-time":"2019-06-04T19:31:19Z","timestamp":1559676679000},"page":"1-15","source":"Crossref","is-referenced-by-count":13,"title":["Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity"],"prefix":"10.1155","volume":"2019","author":[{"given":"S\u00e9bastien","family":"Giraud","sequence":"first","affiliation":[{"name":"INSERM U1082, Poitiers 86000, France"},{"name":"CHU Poitiers, Service de Biochimie, Poitiers 86000, France"},{"name":"Universit\u00e9 de Poitiers, Facult\u00e9 de M\u00e9decine et de Pharmacie, Poitiers 86000, 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