{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T07:12:26Z","timestamp":1787987546319,"version":"build-2784847793"},"reference-count":47,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,7,15]],"date-time":"2019-07-15T00:00:00Z","timestamp":1563148800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gabonese Government"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Infectious Diseases in Obstetrics and Gynecology"],"published-print":{"date-parts":[[2019,7,15]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. Sexually transmitted infections (STIs) are highly prevalent in sub-Saharan Africa. Genital self-sampling may facilitate the screening of STIs in hard-to-reach remote populations far from large health care centers and may increase screening rates. The cross-sectional<jats:italic>GYNAUTO-STI<\/jats:italic>study was carried out to assess the performance of a novel genital veil (V-Veil-Up Gyn Collection Device, V-Veil-Up Pharma, Ltd., Nicosia, Cyprus) as a genital self-sampling device to collect genital secretions to diagnose STIs by molecular biology as compared to reference clinician-collected genital specimens, in adult African women.<jats:italic>Methods<\/jats:italic>. Adult women living in N\u2019Djamena, the capital city of Chad, were recruited from the community and referred to the clinic for women\u2019s sexual health \u201c<jats:italic>La Renaissance Plus<\/jats:italic>\u201d. A clinician obtained an endocervical specimen using flocked swab. Genital secretions were also obtained by self-collection using veil. Both clinician- and self-collected specimens were tested for common curable STIs (including<jats:italic>Chlamydia trachomatis<\/jats:italic>,<jats:italic>Neisseria gonorrhoeae<\/jats:italic>,<jats:italic>Mycoplasma genitalium<\/jats:italic>, and<jats:italic>Trichomonas vaginalis<\/jats:italic>) and genital<jats:italic>Mycoplasma<\/jats:italic>spp. by multiplex real-time PCR (Allplex\u2122 STI Essential Assay, Seegene, Seoul, South Korea). Test positivities for both collection methods were compared by assessing methods agreement, sensitivity, and specificity.<jats:italic>Results<\/jats:italic>. A total of 251 women (mean age, 35.1 years) were prospectively enrolled. Only seven (2.8%) women were found to be infected with at least one common STIs [<jats:italic>C. trachomatis<\/jats:italic>: 3 (1.2%),<jats:italic>N. gonorrhoeae<\/jats:italic>: 1 (0.4%),<jats:italic>M. genitalium<\/jats:italic>: 4 (1.6%) and<jats:italic>T. vaginalis<\/jats:italic>: 1 (0.4%)], while the prevalence of genital mycoplasmas was much higher (54.2%) with a predominance of<jats:italic>Ureaplasma parvum<\/jats:italic>(42.6%). Self-collection by veil was non-inferior to clinician-based collection for genital microorganisms DNA molecular testing, with \u201calmost perfect\u201d agreement between both methods, high sensitivity (97.0%; 95%CI: 92.5-99.2%), and specificity (88.0%; 95%CI: 80.7-93.3%). Remarkably, the mean total number of genital microorganisms detected per woman was 1.14-fold higher in self-collected specimens compared to that in clinician-collected specimens.<jats:italic>Conclusions<\/jats:italic>. Veil-based self-collection of female genital secretions constitutes a convenient tool to collect in gentle way cervicovaginal secretions for accurate molecular detection of genital bacteria. Such sampling procedure could be easily implemented in STIs clinics in sub-Saharan Africa.<\/jats:p>","DOI":"10.1155\/2019\/8639510","type":"journal-article","created":{"date-parts":[[2019,7,15]],"date-time":"2019-07-15T23:31:11Z","timestamp":1563233471000},"page":"1-15","source":"Crossref","is-referenced-by-count":17,"title":["Accuracy of Curable Sexually Transmitted Infections and Genital Mycoplasmas Screening by Multiplex Real-Time PCR Using a Self-Collected Veil among Adult Women in Sub-Saharan Africa"],"prefix":"10.1155","volume":"2019","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0477-2652","authenticated-orcid":true,"given":"Zita Aleyo","family":"Nodjikouambaye","sequence":"first","affiliation":[{"name":"Service de Gyn\u00e9cologie-Obst\u00e9trique, H\u00f4pital de la M\u00e8re et de l\u2019Enfant, N\u2019Djamena, Chad"},{"name":"Ecole Doctorale R\u00e9gionale en Infectiologie Tropicale de Franceville, Franceville, 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