{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T20:30:18Z","timestamp":1740169818567,"version":"3.37.3"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,4,14]],"date-time":"2019-04-14T00:00:00Z","timestamp":1555200000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Colombian Science, Technology and Innovation Institute \u201cFrancisco Jos\u00e9 de Caldas\u201d","award":["073-2016"],"award-info":[{"award-number":["073-2016"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2019,4,14]]},"abstract":"<jats:p>Tuberculosis is an infectious disease caused by<jats:italic>Mycobacterium tuberculosis<\/jats:italic>(<jats:italic>Mtb<\/jats:italic>, i.e., the aetiological agent); the WHO has established this disease as high priority due to its ensuing mortality.<jats:italic>Mtb<\/jats:italic>uses a range of mechanisms for preventing its elimination by an infected host; new, viable alternatives for blocking the host-pathogen interaction are thus sought constantly. This article updates our laboratory\u2019s systematic search for antigens using bioinformatics tools to clarify the<jats:italic>Mtb<\/jats:italic>H37Rv Rv3632 protein\u2019s topology and location. This article reports a C-terminal region consisting of peptides 39255 and 39256 (<jats:sup>81<\/jats:sup>Thr-Arg<jats:sup>114<\/jats:sup>) having high specific binding regarding two infection-related cell lines (A549 and U937); they inhibited mycobacterial entry to U937 cells in a concentration-dependent manner. Rv3632 forms part of the mycobacterial cell envelope, formed by six linear synthetic peptides. Circular dichroism enabled determining the protein\u2019s secondary structure. It was also found that peptide 39254 (<jats:sup>61<\/jats:sup>Gly-Thr<jats:sup>83<\/jats:sup>) was a HABP for alveolar epithelial cells and inhibited mycobacteria entry to these cells regardless of concentration. Sera from active or latent tuberculosis patients did not recognise HABPs 39254 and 39256. These sequences represent a promising approach aiming at their ongoing modification and for including them when designing a multi-epitope, anti-tuberculosis vaccine.<\/jats:p>","DOI":"10.1155\/2019\/8680935","type":"journal-article","created":{"date-parts":[[2019,4,14]],"date-time":"2019-04-14T23:31:38Z","timestamp":1555284698000},"page":"1-13","source":"Crossref","is-referenced-by-count":1,"title":["Specific Binding Peptides from Rv3632: A Strategy for Blocking<i>Mycobacterium tuberculosis<\/i>Entry to Target Cells?"],"prefix":"10.1155","volume":"2019","author":[{"given":"Christian David","family":"S\u00e1nchez-Barinas","sequence":"first","affiliation":[{"name":"Fundaci\u00f3n Instituto de Inmunolog\u00eda de Colombia (FIDIC), Carrera 50 No. 26\u201320, 111321 Bogot\u00e1, Colombia"},{"name":"Universidad del Rosario, Carrera 24 No. 63C-69, 111321 Bogot\u00e1, 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