{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T02:36:40Z","timestamp":1747190200288,"version":"3.40.5"},"reference-count":26,"publisher":"Wiley","license":[{"start":{"date-parts":[[2019,7,30]],"date-time":"2019-07-30T00:00:00Z","timestamp":1564444800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"published-print":{"date-parts":[[2019,7,30]]},"abstract":"<jats:p>Post-kala-azar dermal leishmaniasis (PKDL) is a skin manifestation of visceral leishmaniasis (VL) which develops after apparent cure in some patients. PKDL is considered as the potential reservoir for the VL infection. Molecular epidemiological characterization of<jats:italic>L. donovani<\/jats:italic>isolates obtained from VL and PKDL isolates is essentially required in order to understand the transmission dynamics of the VL infection. To date, genetic variation among the VL and PKDL<jats:italic>L. donovani<\/jats:italic>isolates was not fully elucidated. Therefore, 14 clinical isolates from VL and 4 clinical isolates from PKDL were speciated by<jats:italic>hsp70<\/jats:italic>and rDNA genes. Further characterization of<jats:italic>L. donovani<\/jats:italic>by<jats:italic>haspB<\/jats:italic>PCR demonstrates two different genotypes. All PKDL isolates have the same genetic structure. kDNA PCR-RFLP assay revealed 18 different genotypes; however, structural analysis showed the two distinct kDNA genotype population (<jats:italic>k<\/jats:italic>\u2009=\u20092). The kDNA fingerprint patterns of parasites from hilly districts were clustered separately from low-land districts. Therefore, further study with a large number of samples is urgently required for systematic characterization of the clinical isolates to track the molecular epidemiology of the<jats:italic>Leishmania donovani<\/jats:italic>causing VL and the role of PKDL as a reservoir.<\/jats:p>","DOI":"10.1155\/2019\/9392414","type":"journal-article","created":{"date-parts":[[2019,7,30]],"date-time":"2019-07-30T23:31:50Z","timestamp":1564529510000},"page":"1-8","source":"Crossref","is-referenced-by-count":1,"title":["Application of kDNA Minicircle PCR-RFLP to Characterize<i>Leishmania donovani<\/i>Clinical Isolates Obtained from Post-Kala-Azar Dermal Leishmaniasis in Eastern Nepal"],"prefix":"10.1155","volume":"2019","author":[{"given":"Ojesh","family":"Pokhrel","sequence":"first","affiliation":[{"name":"Department of Microbiology, B. P. 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