{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T13:35:28Z","timestamp":1765546528272,"version":"3.40.5"},"reference-count":22,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T00:00:00Z","timestamp":1582243200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Endocrinology"],"published-print":{"date-parts":[[2020,2,21]]},"abstract":"<jats:p><jats:italic>Introduction<\/jats:italic>. Parathyroid hormone (PTH) is a linear peptide constituted by 84 amino acids and active in its 1\u201384 form, but a wide range of PTH forms produced by its post-transcriptional modifications are present in blood. Many assays with different specificities are commercially available. The aim of our study was to compare a 2<jats:sup>nd<\/jats:sup> and 3<jats:sup>rd<\/jats:sup> generation in healthy population in order to better define the reference range in the healthy population residing in our region. <jats:italic>Materials and Methods<\/jats:italic>. 108 subjects (53 females and 55 males) referring to the transfusion donor were enrolled in the study centre in April 2016 and underwent PTH levels measurements with a 3<jats:sup>rd<\/jats:sup> generation kit (chemiluminescent immunoassay DiaSorin Liaison) and with a 2<jats:sup>nd<\/jats:sup> generation kit (immunoradiometric assay Total Intact PTH Assay (Coated Tube), Scantibodies). Also calcium, phosphate, creatinine, and 25OHD3 were measured. A questionnaire on lifestyle and dietary habits was obtained. <jats:italic>Results<\/jats:italic>. The median PTH values obtained with the 2<jats:sup>nd<\/jats:sup> generation assay and the whole 3<jats:sup>rd<\/jats:sup> generation assay were 20.26\u2009pg\/ml and 23.11\u2009pg\/ml, respectively. Bland\u2013Altman method showed substantial concordance between the two PTH assays, although with an overestimation of the 3<jats:sup>rd<\/jats:sup> generation method over the 2<jats:sup>nd<\/jats:sup> generation method. There was no correlation between 3<jats:sup>rd<\/jats:sup> generation PTH and 25OHD3 and creatinine. Calcium was negatively correlated with PTH only when measured with 3<jats:sup>rd<\/jats:sup> generation kit. <jats:italic>Conclusions<\/jats:italic>. On the basis of our data, obtained from healthy subjects, we can conclude that the reference range used by our laboratory was too narrow and was necessary to reestablish normal ranges according to our population. This is useful to avoid hyperparathyroidism misdiagnosis.<\/jats:p>","DOI":"10.1155\/2020\/1053719","type":"journal-article","created":{"date-parts":[[2020,2,21]],"date-time":"2020-02-21T18:30:37Z","timestamp":1582309837000},"page":"1-7","source":"Crossref","is-referenced-by-count":6,"title":["PTH: Redefining Reference Ranges in a Healthy Population\u2014The Role of Interfering Factors and the Type of Laboratory Assay"],"prefix":"10.1155","volume":"2020","author":[{"given":"Simona","family":"Censi","sequence":"first","affiliation":[{"name":"Endocrinology Unit, Department of Medicine (DIMED), University of Padua, Padua, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6057-4642","authenticated-orcid":true,"given":"Maurizio","family":"Iacobone","sequence":"additional","affiliation":[{"name":"Endocrine Surgery Unit, Department of Surgery, Oncology and Gastroenterology (DiSCOG), University of Padua, Padua, 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