{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T04:42:22Z","timestamp":1778042542923,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,12]],"date-time":"2020-11-12T00:00:00Z","timestamp":1605139200000},"content-version":"vor","delay-in-days":316,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007452","name":"Wu Jieping Medical Foundation","doi-asserted-by":"publisher","award":["320.6750.18272"],"award-info":[{"award-number":["320.6750.18272"]}],"id":[{"id":"10.13039\/100007452","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. The abnormal vascular permeability is associated with the formation of chronic rhinosinusitis with nasal polyps (CRSwNP). Previously, our study demonstrated that the nasal lavage fluid\u2010 (NLF\u2010) derived exosomes from CRSwNP can promote the vascular permeability of human umbilical vein endothelial cells (HUVECs). miR\u201022\u20103p, a specific differentiated miRNA, is reported to regulate microvessels in some diseases. This study is purposed to explore the impact of exosomal miR\u201022\u20103p derived from CRSwNP on vascular permeability and identify the underlying targets. <jats:italic>Methods<\/jats:italic>. Exosomes were extracted from NLF of 26 CRSwNP patients and 10 control patients. Quantitative real\u2010time PCR (qRT\u2010 PCR) was applied to evaluate the relative level of exosomal miR\u201022\u20103p. The impact of exosomal miR\u201022\u20103p on HUVECs was assessed by permeability assays in vitro. The potential molecular targets of miR\u201022\u20103p were investigated by applying such technologies as dual\u2010luciferase reporter assay and western blot. <jats:italic>Results<\/jats:italic>. miR\u201022\u20103p was upregulated in NLF\u2010derived exosomes from CRSwNP. Exosomal miR\u201022\u20103p derived from CRSwNP enhanced the tubule permeability of HUVECs. Vascular endothelial\u2010 (VE\u2010) cadherin (<jats:italic>CDH5<\/jats:italic>) was identified as a direct target of miR\u201022\u20103p. miR\u201022\u20103p regulated the vascular permeability by targeting VE\u2010cadherin in HUVECs. <jats:italic>Conclusions<\/jats:italic>. Exosomal miR\u201022\u20103p derived from NLF of CRSwNP plays an important role in regulating vascular permeability by targeting VE\u2010cadherin.<\/jats:p>","DOI":"10.1155\/2020\/1237678","type":"journal-article","created":{"date-parts":[[2020,11,13]],"date-time":"2020-11-13T01:05:14Z","timestamp":1605229514000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Exosomal miR\u201022\u20103p Derived from Chronic Rhinosinusitis with Nasal Polyps Regulates Vascular Permeability by Targeting VE\u2010Cadherin"],"prefix":"10.1155","volume":"2020","author":[{"given":"Wei","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongbing","family":"Yan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yinyin","family":"Pei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Yao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3602-5264","authenticated-orcid":false,"given":"Bo","family":"You","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5205-1091","authenticated-orcid":false,"given":"Jing","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,11,12]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.4193\/Rhin20.401"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2020.12.2.176"},{"key":"e_1_2_10_3_2","doi-asserted-by":"publisher","DOI":"10.4193\/rhino50e2"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-013-1583-0"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/all.12277"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1167\/iovs.16-19805"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41388-018-0183-6"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-018-0425-0"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1177\/1753425918785715"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-019-1806-6"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2018.8634"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-9-9"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1596"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.23.090506.123406"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.00826"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2015.7.5.449"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1166\/jnn.2015.9161"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22230"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.03.030"},{"key":"e_1_2_10_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0020291"},{"key":"e_1_2_10_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/1873-3468.12565"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2019.03.026"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1521230113"},{"key":"e_1_2_10_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/smll.201101708"},{"key":"e_1_2_10_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.05.051"},{"key":"e_1_2_10_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201343376"},{"key":"e_1_2_10_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/all.12374"},{"key":"e_1_2_10_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-016-0927-4"},{"key":"e_1_2_10_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07810-w"},{"key":"e_1_2_10_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2017.105"},{"key":"e_1_2_10_32_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29920"},{"key":"e_1_2_10_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088685"},{"key":"e_1_2_10_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep44584"},{"key":"e_1_2_10_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2009.01.035"},{"key":"e_1_2_10_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2013.08.020"},{"key":"e_1_2_10_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394311-8.00006-6"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/1237678.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2020\/1237678.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2020\/1237678","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T11:23:00Z","timestamp":1735644180000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2020\/1237678"}},"subtitle":[],"editor":[{"given":"Giulio","family":"Gasparini","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":37,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1155\/2020\/1237678"],"URL":"https:\/\/doi.org\/10.1155\/2020\/1237678","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2020-06-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-12","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1237678"}}