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Bacterial vaginosis (BV), one of the most common vaginal ecosystem-related microbiologic syndromes, is the most common disorder in women of reproductive age. <jats:italic>Gardnerella<\/jats:italic> (<jats:italic>G.<\/jats:italic>) <jats:italic>vaginalis<\/jats:italic> is the predominant species causing this infection. Our aim was to compare the antimicrobial susceptibilities of metronidazole and clindamycin against <jats:italic>G. vaginalis<\/jats:italic> at planktonic and biofilm levels. <jats:italic>Methods<\/jats:italic>. From September 2019 to October 2019, we recruited a total of 10 patients with BV who underwent gynecological examinations at Beijing Obstetrics and Gynecology Hospital. <jats:italic>G. vaginalis<\/jats:italic> isolates were obtained from the vagina and identified using their characteristic colony morphology. Sequence data of clinical <jats:italic>G. vaginalis<\/jats:italic> isolates were confirmed by comparing 16S rDNA sequences. Subsequently, clinical isolates were evaluated for antimicrobial susceptibilities <jats:italic>in vitro<\/jats:italic> to metronidazole and clindamycin at planktonic and biofilm levels. The minimum inhibitory concentration (MIC) for metronidazole and clindamycin was evaluated by antimicrobial susceptibility testing. The minimum biofilm eradication concentration (MBEC) was evaluated by the biofilm inhibition assay. <jats:italic>Results<\/jats:italic>. Planktonic clinical isolates showed a significantly higher susceptibility rate (76.67%) and lower resistance rate (23.33%) to clindamycin than to metronidazole (susceptibility rate: 38.24%; resistance rate: 58.82%; <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math> for both). Furthermore, in comparison to planktonic isolates, the minimum inhibitory concentration (MIC) of metronidazole was significantly higher for biofilm-forming isolates (7.3\u2009\u00b1\u20092.6\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL vs. 72.4\u2009\u00b1\u200918.3\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL; <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.005<\/mml:mn><\/mml:math>); the resistance rate was 27.3%, and the minimum biofilm eradication concentration (MBEC) was &gt;128\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL. Moreover, the MIC of clindamycin was higher too for biofilm-forming isolates (0.099\u2009\u00b1\u20090.041\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL vs. 23.7\u2009\u00b1\u20099.49\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL; <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.034<\/mml:mn><\/mml:math>); the resistance rate was 27.3%, and the MBEC of clindamycin was 28.4\u2009\u00b1\u20096.50\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL. <jats:italic>Conclusion<\/jats:italic>. Our results indicate that in comparison to metronidazole, clindamycin seems to be a better choice to tackle <jats:italic>G. vaginalis<\/jats:italic> as it exhibits a relatively higher susceptibility rate and lower resistance rate.<\/jats:p>","DOI":"10.1155\/2020\/1361825","type":"journal-article","created":{"date-parts":[[2020,6,16]],"date-time":"2020-06-16T23:30:38Z","timestamp":1592350238000},"page":"1-7","source":"Crossref","is-referenced-by-count":35,"title":["Antimicrobial Susceptibility Testing of Metronidazole and Clindamycin against <i>Gardnerella vaginalis<\/i> in Planktonic and Biofilm Formation"],"prefix":"10.1155","volume":"2020","author":[{"given":"Ting","family":"Li","sequence":"first","affiliation":[{"name":"Department of Gynecology, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing 100026, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department 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