{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T13:03:16Z","timestamp":1769000596542,"version":"3.49.0"},"reference-count":60,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T00:00:00Z","timestamp":1588204800000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010883","name":"Traditional Chinese Medicine Bureau of Guangdong Province","doi-asserted-by":"publisher","award":["20181102"],"award-info":[{"award-number":["20181102"]}],"id":[{"id":"10.13039\/501100010883","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010883","name":"Traditional Chinese Medicine Bureau of Guangdong Province","doi-asserted-by":"publisher","award":["201704020213"],"award-info":[{"award-number":["201704020213"]}],"id":[{"id":"10.13039\/501100010883","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangzhou Science and Technology Project","award":["20181102"],"award-info":[{"award-number":["20181102"]}]},{"name":"Guangzhou Science and Technology Project","award":["201704020213"],"award-info":[{"award-number":["201704020213"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Canadian Journal of Infectious Diseases and Medical Microbiology"],"published-print":{"date-parts":[[2020,4,30]]},"abstract":"<jats:p><jats:italic>Streptococcus agalactiae<\/jats:italic> colonization in pregnant women can cause postpartum intrauterine infections and life-threatening neonatal infections. To formulate strategies for the prevention and treatment of <jats:italic>S. agalactiae<\/jats:italic> infections, we performed a comprehensive analysis of antibiotic resistance and a molecular-based epidemiological investigation of <jats:italic>S. agalactiae<\/jats:italic> in this study. Seventy-two <jats:italic>S. agalactiae<\/jats:italic> strains, collected from pregnant women, were subjected to antibiotic susceptibility tests; then, the screened erythromycin and clindamycin nonsusceptible isolates were used for macrolides and clindamycin resistance genes detection, respectively. Detection of resistance genes, serotyping, and determination of virulence genes were performed by polymerase chain reaction. The clonal relationships among the colonized strains were evaluated by multilocus sequence typing. Matrix-assisted laser desorption\/ionization time-of-flight mass spectrometry (MALDI-TOF MS) mass peak analysis was performed to discriminate the specific sequence types (STs). In our study, 69.4% and 47.2% of the strains were nonsusceptible to erythromycin and clindamycin, respectively; the multidrug resistance rate was 66.7%. All erythromycin nonsusceptible strains harbored resistance genes, whereas only 52.9% of the clindamycin nonsusceptible strains possessed the <jats:italic>linB<\/jats:italic> gene. Erythromycin resistance was mainly mediated by the <jats:italic>ermB<\/jats:italic> or <jats:italic>mefA\/E<\/jats:italic> genes. Four serotypes were identified, and the most common serotype was serotype III (52.8%), followed by Ib (22.2%), Ia (18.0%), and II (4.2%). All the strains were divided into 18 STs that were assigned to nine clonal complexes. Most of the major STs were distributed into specific serotypes, including ST19\/serotype III, ST17\/serotype III, ST485\/serotype Ia, ST862\/serotype III, and ST651\/serotype III. Analysis of virulence genes yielded seven clusters, of which <jats:italic>bca<\/jats:italic>-<jats:italic>cfb<\/jats:italic>-<jats:italic>scpB<\/jats:italic>-<jats:italic>lmb<\/jats:italic> (61.6%) was the predominant virulence gene cluster. Among all ST strains distributed in this region, only the ST17 strains had a mass peak at 7620\u2009Da. The outcomes of this study are beneficial for the epidemiological comparison of colonized <jats:italic>S. agalactiae<\/jats:italic> in different regions and may be helpful for developing the strategies for the prevention of <jats:italic>S. agalactiae<\/jats:italic> infection in Guangzhou. Furthermore, our results show that MALDI-TOF MS can be used for the rapid identification of the ST17 strains.<\/jats:p>","DOI":"10.1155\/2020\/1368942","type":"journal-article","created":{"date-parts":[[2020,4,30]],"date-time":"2020-04-30T23:30:42Z","timestamp":1588289442000},"page":"1-11","source":"Crossref","is-referenced-by-count":16,"title":["Antibiotic Resistance and Molecular Epidemiological Characteristics of <i>Streptococcus agalactiae<\/i> Isolated from Pregnant Women in Guangzhou, South China"],"prefix":"10.1155","volume":"2020","author":[{"given":"Zhaomin","family":"Cheng","sequence":"first","affiliation":[{"name":"The Second Clinical College, Guangzhou University of Chinese Medicine, Guangzhou, China"},{"name":"Department of Laboratory Medicine, Guangdong Provincial Hospital of Chinese Medicine, Guangzhou, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pinghua","family":"Qu","sequence":"additional","affiliation":[{"name":"The Second Clinical College, 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