{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:29:41Z","timestamp":1790771381391,"version":"4.1.0"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T00:00:00Z","timestamp":1604966400000},"content-version":"vor","delay-in-days":314,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31972599"],"award-info":[{"award-number":["31972599"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>\n                    <jats:italic>Purpose<\/jats:italic>\n                    .\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010Defensin 118 (DEFB118) is a novel host defense peptide (HDP) identified in humans. To evaluate its potentials for future utilization, the DEFB118 gene was expressed in\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (\n                    <jats:italic>E. coli<\/jats:italic>\n                    ) and the recombinant protein was fully characterized.\n                    <jats:italic>Methods<\/jats:italic>\n                    . The DEFB118 protein was obtained by heterologous expression using\n                    <jats:italic>E. coli<\/jats:italic>\n                    Rosetta (DE3). Antibacterial activity of DEFB118 was determined by using various bacterial strains. IPEC\u2010J cells challenged by\n                    <jats:italic>E. coli<\/jats:italic>\n                    K88 were used to determine its influences on inflammatory responses.\n                    <jats:italic>Results<\/jats:italic>\n                    . The\n                    <jats:italic>E. coli<\/jats:italic>\n                    transformants yielded more than 250\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL DEFB118 protein after 4\u2009h induction by 1.0\u2009mM IPTG. The DEFB118 was estimated by SDS\u2010PAGE to be 30\u2009kDa, and MALDI\u2010TOF analysis verified that it is a human\n                    <jats:italic>\u03b2<\/jats:italic>\n                    \u2010defensin 118. Importantly, the DEFB118 showed antimicrobial activities against both Gram\u2010negative bacteria (\n                    <jats:italic>E. coli K88<\/jats:italic>\n                    and\n                    <jats:italic>E. coli DH5\u03b1<\/jats:italic>\n                    ) and Gram\u2010positive bacteria (\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and\n                    <jats:italic>B. subtilis<\/jats:italic>\n                    ), with a minimum inhibitory concentration (MIC) of 4\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL. Hemolytic assays showed that DEFB118 had no detrimental impact on cell viability. Additionally, DEFB118 was found to elevate the viability of IPEC\u2010J2 cells upon\n                    <jats:italic>E. coli<\/jats:italic>\n                    K88 challenge. Moreover, DEFB118 significantly decreased cell apoptosis in the late apoptosis phase and downregulated the expression of inflammatory cytokines such as IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    and TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    in IPEC\u2010J2 cell exposure to\n                    <jats:italic>E. coli<\/jats:italic>\n                    K88.\n                    <jats:italic>Conclusions<\/jats:italic>\n                    . These results suggested a novel function of the mammalian defensins, and the antibacterial and anti\u2010inflammatory properties of DEFB118 may allow it as a potential substitute for conventionally used antibiotics or drugs.\n                  <\/jats:p>","DOI":"10.1155\/2020\/1395304","type":"journal-article","created":{"date-parts":[[2020,11,10]],"date-time":"2020-11-10T21:50:17Z","timestamp":1605045017000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Expression and Functional Characterization of a Novel Antimicrobial Peptide: Human Beta\u2010Defensin 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