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About 15 million people worldwide suffer strokes each year and 5 million people are left with permanent disabilities which is due to the loss of local blood supply to the brain, resulting in a neurologic deficit. <jats:italic>Panax notoginseng<\/jats:italic> (Bruk.) F. H. Chen (Araliaceae) is a traditional Chinese herbal medicine widely used in the treatment of cardio\u2010cerebrovascular diseases. <jats:italic>Objective<\/jats:italic>. This study investigated whether <jats:italic>Panax notoginseng<\/jats:italic> saponins (PNS) extracted from <jats:italic>Panax notoginseng<\/jats:italic> (Bruk.) F. H. Chen played a neuroprotective role by affecting the EGFR\/PI3K\/AKT pathway in oxygen\u2010glucose deprived (OGD) SH\u2010SY5Y cells. <jats:italic>Materials and Methods<\/jats:italic>. Different groups of OGD SH\u2010SY5Y cells were treated with varying doses of PNS, PNS\u2009+\u2009AG1478 (a specific inhibitor of EGFR), or AG1478 for 16 hours. CCK8, Annexin V\u2010FITC\/PI apoptosis analysis, and LDH release analysis were used to determine cell viability, apoptosis rate, and amounts of LDH. Quantitative real\u2010time PCR (q\u2010RT\u2010PCR) and western blotting were used to measure mRNA and proteins levels of p\u2010EGFR\/EGFR, p\u2010PI3K\/PI3K, and p\u2010AKT\/AKT in SH\u2010SY5Y cells subjected to OGD. <jats:italic>Results<\/jats:italic>. PNS significantly enhanced cell viability, reduced apoptosis, and weakened cytotoxicity by inhibiting the release of LDH. The mRNA expression profiles of EGFR, PI3K, and AKT showed no difference between model and other groups. Additionally, ratios of p\u2010EGFR, p\u2010PI3K, and p\u2010AKT to EGFR, PI3K, and AKT proteins expression, respectively, all increased significantly. <jats:italic>Conclusions<\/jats:italic>. These findings indicate that PNS enhanced neuroprotective effects by activating the EGFR\/PI3K\/AKT pathway and elevating phosphorylation levels in OGD SH\u2010SY5Y cells.<\/jats:p>","DOI":"10.1155\/2020\/1403572","type":"journal-article","created":{"date-parts":[[2020,7,31]],"date-time":"2020-07-31T23:30:46Z","timestamp":1596238246000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["In Vitro Evaluation of the Neuroprotective Effect of <i>Panax notoginseng<\/i> Saponins by Activating the EGFR\/PI3K\/AKT Pathway"],"prefix":"10.1155","volume":"2020","author":[{"given":"Shuang","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tiantian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kai","family":"Cen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4003-8244","authenticated-orcid":false,"given":"Yihuai","family":"Zou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaowei","family":"Shi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dongrui","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3771-3528","authenticated-orcid":false,"given":"Yonghong","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9207-1169","authenticated-orcid":false,"given":"Limin","family":"Chai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8237-1734","authenticated-orcid":false,"given":"Yizhou","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yikun","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2852-5648","authenticated-orcid":false,"given":"Lingqun","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,7,31]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)31012-1"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(13)61097-1"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.116.025250"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.2147\/VHRM.S63791"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/204840"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2017.00702"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b13-00770"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.06.017"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2013.09.004"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1177\/1759091415602463"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2015.76"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-014-0135-3"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2015.1012157"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2017.5878"},{"key":"e_1_2_11_15_2","first-page":"2643","article-title":"Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture","volume":"33","author":"Chen J. 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