{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T22:52:39Z","timestamp":1791499959539,"version":"4.3.4"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,11,20]],"date-time":"2020-11-20T00:00:00Z","timestamp":1605830400000},"content-version":"vor","delay-in-days":324,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004561","name":"Ministry of Education and Science of the Republic of Kazakhstan","doi-asserted-by":"publisher","award":["AP05131463"],"award-info":[{"award-number":["AP05131463"]}],"id":[{"id":"10.13039\/501100004561","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>In this paper, we first used recombinant influenza viral vector (rIVV) subtype H5N1 expressing from the open reading frame of NS1 80 and NS1 124 amino acids of <jats:italic>Brucella<\/jats:italic> outer membrane proteins (Omp) 16 and 19, ribosomal L7\/L12, and Cu\u2010Zn superoxide dismutase (SOD) proteins to develop a human brucellosis vaccine. We made 18 combinations of IVVs in mono\u2010, bi\u2010, and tetravalent vaccine formulations and tested them on mice to select the safest and most effective vaccine samples. Then, the most effective vaccine candidates were further tested on guinea pigs. Safety of the rIVV\u2010based vaccine candidate was evaluated by a mouse weight\u2010gain test. Mice and guinea pigs were challenged with the virulent strain <jats:italic>B. melitensis<\/jats:italic> 16M. The protective effect of the rIVV\u2010based vaccine candidate was assessed by quantitation of <jats:italic>Brucella<\/jats:italic> colonization in tissues and organs of challenged animals. All vaccine formulations were safe in mice. Tested vaccine formulations, as well as the commercial <jats:italic>B. melitensis<\/jats:italic> Rev.1 vaccine, have been found to protect mice from <jats:italic>B. melitensis<\/jats:italic> 16M infection within the range of 1.6 to 2.97 log<jats:sub>10<\/jats:sub> units (<jats:italic>P<\/jats:italic> &lt; 0.05). Tetravalent vaccine formulations from the position of NS1 80 amino acids (0.2 \u00b1 0.4), as well as the commercial <jats:italic>B. melitensis<\/jats:italic> Rev.1 vaccine (1.2 \u00b1 2.6), have been found to protect guinea pigs from <jats:italic>B. melitensis<\/jats:italic> 16M infection at a significant level (<jats:italic>P<\/jats:italic> &lt; 0.05). Thus, tetravalent vaccine formulation Flu\u2010NS1\u201080\u2010Omp16+Flu\u2010NS1\u201080\u2010L7\/L12+Flu\u2010NS1\u201080\u2010Omp19+Flu\u2010NS1\u201080\u2010SOD was chosen as a potential vaccine candidate for further development of an effective human vaccine against brucellosis. These results show a promising future for the development of a safe human vaccine against brucellosis based on rIVVs.<\/jats:p>","DOI":"10.1155\/2020\/1438928","type":"journal-article","created":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T01:50:06Z","timestamp":1605923406000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Development of Human Vectored Brucellosis Vaccine Formulation: Assessment of Safety and Protectiveness of Influenza Viral Vectors Expressing <i>Brucella<\/i> Immunodominant Proteins in Mice and Guinea Pigs"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1891-2748","authenticated-orcid":false,"given":"Dina","family":"Bugybayeva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6538-8486","authenticated-orcid":false,"given":"Sholpan","family":"Ryskeldinova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3536-5483","authenticated-orcid":false,"given":"Nadezhda","family":"Zinina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0650-7457","authenticated-orcid":false,"given":"Makhpal","family":"Sarmykova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7267-3931","authenticated-orcid":false,"given":"Nurika","family":"Assanzhanova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9247-6273","authenticated-orcid":false,"given":"Zhailaubay","family":"Kydyrbayev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5823-1280","authenticated-orcid":false,"given":"Kaissar","family":"Tabynov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,11,20]]},"reference":[{"key":"e_1_2_10_1_2","first-page":"233","article-title":"Brucellosis in humans-etiology, diagnostics, clinical forms","volume":"20","author":"Gali\u0144ska E. 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