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Visceral pain is one of the most common types of pain and particularly in the abdomen is associated with gastrointestinal diseases. Bulleyaconitine A (BAA), isolated from <jats:italic>Aconitum bulleyanum<\/jats:italic>, is prescribed in China to treat chronic pain. The present study is aimed at evaluating the mechanisms underlying BAA visceral antinociception. <jats:italic>Methods<\/jats:italic>. The rat model of chronic visceral hypersensitivity was set up by colonic perfusion of 2,4,6-trinitrobenzene sulfonic acid (TNBS) on postnatal day 10 with coapplication of heterotypic intermittent chronic stress (HeICS). <jats:italic>Results<\/jats:italic>. The rat model of chronic visceral hypersensitivity exhibited remarkable abdominal withdrawal responses and mechanical hyperalgesia in hind paws, which were dose-dependently attenuated by single subcutaneous of administration of BAA (30 and 90\u2009<jats:italic>\u03bc<\/jats:italic>g\/kg). Pretreatment with the microglial inhibitor minocycline, dynorphin A antiserum, and <jats:italic>\u03ba<\/jats:italic>-opioid receptor antagonist totally blocked BAA-induced visceral antinociception and mechanical antihyperalgesia. Spontaneous excitatory postsynaptic currents (sEPSCs) in spinal dorsal horn lamina II neurons were recorded by using whole-cell patch clamp. Its frequency (but not amplitude) from TNBS-treated rats was remarkably higher than that from na\u00efve rats. BAA (1\u2009<jats:italic>\u03bc<\/jats:italic>M) significantly reduced the frequency of sEPSCs from TNBS-treated rats but not na\u00efve rats. BAA-inhibited spinal synaptic plasticity was blocked by minocycline, the dynorphin A antiserum, and <jats:italic>\u03ba<\/jats:italic>-opioid receptor antagonist. Dynorphin A also inhibited spinal synaptic plasticity in a <jats:italic>\u03ba<\/jats:italic>-opioid receptor-dependent manner. <jats:italic>Conclusions<\/jats:italic>. These results suggest that BAA produces visceral antinociception by stimulating spinal microglial release of dynorphin A, which activates presynaptic <jats:italic>\u03ba<\/jats:italic>-opioid receptors in afferent neurons and inhibits spinal synaptic plasticity, highlighting a novel interaction mode between microglia and neurons.<\/jats:p>","DOI":"10.1155\/2020\/1484087","type":"journal-article","created":{"date-parts":[[2020,5,23]],"date-time":"2020-05-23T23:30:21Z","timestamp":1590276621000},"page":"1-13","source":"Crossref","is-referenced-by-count":14,"title":["Bulleyaconitine A Inhibits Visceral Nociception and Spinal Synaptic Plasticity through Stimulation of Microglial Release of Dynorphin A"],"prefix":"10.1155","volume":"2020","author":[{"given":"Sheng-Nan","family":"Huang","sequence":"first","affiliation":[{"name":"Shanghai Key Laboratory of Psychotic Disorders, Shanghai Mental Health Center, Shanghai Jiao Tong University School of Medicine, Shanghai, 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