{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:47:45Z","timestamp":1787017665413,"version":"build-2736575974"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T00:00:00Z","timestamp":1579651200000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81460198"],"award-info":[{"award-number":["81460198"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31260233"],"award-info":[{"award-number":["31260233"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>\n                    <jats:italic>Gastrodia elata<\/jats:italic>\n                    is a traditional herbal medicine that has been used for centuries to treat rheumatism. Previous studies have confirmed that ethanol extracts of\n                    <jats:italic>Gastrodia elata<\/jats:italic>\n                    have anti\u2010inflammatory and antioxidant activities, and the\n                    <jats:italic>n<\/jats:italic>\n                    \u2010butanol fraction exerts a higher inhibitory effect. However, the in vivo anti\u2010inflammatory effects of\n                    <jats:italic>Gastrodia elata<\/jats:italic>\n                    have not been evaluated. Thus, we assessed the therapeutic effect of the\n                    <jats:italic>n<\/jats:italic>\n                    \u2010butanol extract of\n                    <jats:italic>Gastrodia elata<\/jats:italic>\n                    (BGE) on complete Freund\u2019s adjuvant\u2010 (CFA\u2010) induced arthritis rats which were separated into six groups (NOR; MODEL; CFA\u2009+\u2009dexamethasone (DEX); CFA\u2009+\u200925, 50, 100\u2009mg\/kg BGE). The paw swelling, joint radiology, and histology were used to analyze the effect of BGE on delaying the progression of rheumatoid arthritis. Furthermore, serum levels of inflammatory cytokines were analyzed via ELISA. In addition, the effect of BGE on nitric oxide (NO) production, expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase\u20102(COX\u20102), and inflammatory cytokines were detected in lipopolysaccharide\u2010 (LPS\u2010) stimulated RAW264.7 macrophage cells. Lastly, the impacts of BGE on the activation of the mitogen\u2010activated protein kinases (MAPK) pathway in CFA rats and LPS\u2010stimulated RAW264.7 macrophage were examined by western blot analysis. The results show that BGE can significantly reduce paw swelling without losing the body weight of rats. Imaging assessment confirms that BGE can protect cartilage from destruction, as well as reducing inflammatory cell infiltration and synovial proliferation. Moreover, BGE suppresses the production of inflammatory cytokines in serum and inhibits the activation of the phosphorylation of p38 and ERK in CFA rats. BGE was also demonstrated to decrease the production of NO and inflammatory cytokines in LPS\u2010stimulated RAW264.7 cells. The effect of BGE in LPS\u2010induced expression leads to reduced p38 and ERK phosphorylation and also downregulates the protein expression of iNOS and COX\u20102. Taken together, BGE exhibits a potential therapeutic effect on CFA rats, and its anti\u2010inflammatory and antioxidant effects were possibly exerted by regulation of ERK\/p38MAPK.\n                  <\/jats:p>","DOI":"10.1155\/2020\/1658618","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T18:30:52Z","timestamp":1579717852000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["<i>N<\/i>\n                    \u2010Butanol Extract of\n                    <i>Gastrodia elata<\/i>\n                    Suppresses Inflammatory Responses in Lipopolysaccharide\u2010Stimulated Macrophages and Complete Freund\u2019s Adjuvant\u2010 (CFA\u2010) Induced Arthritis Rats via Inhibition of MAPK Signaling Pathway"],"prefix":"10.1155","volume":"2020","author":[{"given":"Peng","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changzhao","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianping","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houjie","family":"Dai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3122-1370","authenticated-orcid":false,"given":"Yan","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,22]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2010.12.006"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17091410"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.27584"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.124"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2019.111864"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kes113"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2015.171"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-013-0014-6"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.berh.2014.10.017"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3495"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.12703\/p6-31"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-019-04577-3"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.5455\/medarh.2017.71.148-150"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2018.00914"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/5308405"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1142\/s0192415x05002631"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24224033"},{"key":"e_1_2_9_18_2","article-title":"Effects of total flavonoids from exocarpium citri grandis on air pollution particle-induced pulmonary inflammation and oxidative stress in mice","volume":"24","author":"Zhu Z.","year":"2019","journal-title":"Journal of Food Science"},{"key":"e_1_2_9_19_2","first-page":"223","article-title":"Comparative anti-arthritic investigation of iridoid glycosides and crocetin derivatives from Gardenia jasminoides Ellis in Freund\u2019s complete adjuvant-induced arthritis in rats","volume":"7","author":"Hu Y.","year":"2018","journal-title":"Phytomedicine"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2016.06.057"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24132436"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/516741"},{"key":"e_1_2_9_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2009.10.009"},{"key":"e_1_2_9_24_2","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.28.1016"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2016.11.136"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2006.10.006"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.1142\/s0192415x09006916"},{"key":"e_1_2_9_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2018.08.011"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1296.186346"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.1155\/2008\/129873"},{"key":"e_1_2_9_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2017.02.006"},{"key":"e_1_2_9_32_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867323666160506125218"},{"key":"e_1_2_9_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2015.03.047"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.1042\/cs1040027"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar2333"},{"key":"e_1_2_9_36_2","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/4606459"},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1524-6175.2006.06025.x"},{"key":"e_1_2_9_38_2","first-page":"787","article-title":"The effect of Cistanche deserticola poly saccharides (CDPS) on macrophages activation","volume":"25","author":"Wang X. Y.","year":"2009","journal-title":"Chinese Pharmacology Bulletin"},{"key":"e_1_2_9_39_2","doi-asserted-by":"publisher","DOI":"10.1248\/yakushi.16-00027"},{"key":"e_1_2_9_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-016-0396-1"},{"key":"e_1_2_9_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.07.047"},{"key":"e_1_2_9_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-857X(03)00073-5"},{"key":"e_1_2_9_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.24781"},{"key":"e_1_2_9_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/bf02973905"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2020\/1658618.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2020\/1658618.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2020\/1658618","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T02:59:57Z","timestamp":1722999597000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2020\/1658618"}},"subtitle":[],"editor":[{"given":"Jairo Kennup","family":"Bastos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1155\/2020\/1658618"],"URL":"https:\/\/doi.org\/10.1155\/2020\/1658618","archive":["Portico"],"relation":{},"ISSN":["1741-427X","1741-4288"],"issn-type":[{"value":"1741-427X","type":"print"},{"value":"1741-4288","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2019-08-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-12-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1658618"}}