{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,22]],"date-time":"2026-06-22T04:30:32Z","timestamp":1782102632754,"version":"3.54.5"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,9,30]],"date-time":"2020-09-30T00:00:00Z","timestamp":1601424000000},"content-version":"vor","delay-in-days":273,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004569","name":"Ministerstwo Edukacji i Nauki","doi-asserted-by":"publisher","award":["DS124"],"award-info":[{"award-number":["DS124"]}],"id":[{"id":"10.13039\/501100004569","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>\n                    Ovarian cancer (OC) is one of the deadliest gynecological cancers. Recent studies suggest a crucial role of inflammatory immune system cells in the progression and metastasis of OC. The understanding of inflammatory mechanisms is pivotal for the selection of a biomarker that allows the differentiation between malignant and benign tumors, monitoring the progression of the disease, and identification of patients that will respond to implemented treatment. Our study is aimed at evaluating the profile of IL\u20106 in the plasma and peritoneal fluid (PF) of patients with various clinical manifestations of OC (\n                    <jats:italic>n<\/jats:italic>\n                    = 78). We also examined the relationship between IL\u20106 and PD\u2010L1\/PD\u2010L2 positive CD45\n                    <jats:sup>+<\/jats:sup>\n                    CD14\n                    <jats:sup>+<\/jats:sup>\n                    inflammatory cell (MO\/MA) levels in three OC environments (TME): peripheral blood (PB), PF, and tumor (TT) and their clinical and prognostic relevance in OC patients. The expression of PD\u2010L1\/PD\u2010L2 molecules was analyzed by flow cytometry. The IL\u20106 levels were determined by ELISA. We found an elevated level of PD\u2010L1\/PD\u2010L2 positive MO\/MA in TT compared to PB (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.0001). Significantly higher (\n                    <jats:italic>p<\/jats:italic>\n                    &lt; 0.0001) levels of IL\u20106 were observed in PF of the OC patients than in the benign ovarian tumor group (\n                    <jats:italic>n<\/jats:italic>\n                    = 31). Additionally, we found higher IL\u20106 levels in PF than in the plasma of the OC patients. Interestingly, accumulation of IL\u20106 was observed in PF of patients with low\u2010differentiated OC and correlated with worse prognosis. Moreover, we observed correlations between the level of IL\u20106 and CD45\n                    <jats:sup>+<\/jats:sup>\n                    CD14\n                    <jats:sup>+<\/jats:sup>\n                    cells and between CD45\n                    <jats:sup>+<\/jats:sup>\n                    CD14\n                    <jats:sup>+<\/jats:sup>\n                    PD\u2010L1\n                    <jats:sup>+<\/jats:sup>\n                    cells and the IL\u20106 level in PF. For the first time, we discovered that the higher percentage of CD45\n                    <jats:sup>+<\/jats:sup>\n                    CD14\n                    <jats:sup>+<\/jats:sup>\n                    PD\u2010L2\n                    <jats:sup>+<\/jats:sup>\n                    cells in PF predicts better survival of OC patients. Our study suggests that CD45\n                    <jats:sup>+<\/jats:sup>\n                    CD14\n                    <jats:sup>+<\/jats:sup>\n                    PD\u2010L2\n                    <jats:sup>+<\/jats:sup>\n                    cells and IL\u20106 may be predictive biomarkers for OC patients. Understanding how the composition of TME changes during OC development and progression is a prerequisite for projecting new therapeutic strategies. Overall, further validation research is warranted.\n                  <\/jats:p>","DOI":"10.1155\/2020\/1715064","type":"journal-article","created":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T08:07:54Z","timestamp":1601539674000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Prognostic and Clinical Value of Interleukin 6 and CD45\n                    <sup>+<\/sup>\n                    CD14\n                    <sup>+<\/sup>\n                    Inflammatory Cells with PD\u2010L1\n                    <sup>+<\/sup>\n                    \/PD\u2010L2\n                    <sup>+<\/sup>\n                    Expression in Patients with Different Manifestation of Ovarian Cancer"],"prefix":"10.1155","volume":"2020","author":[{"given":"Iwona","family":"Wertel","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1022-3323","authenticated-orcid":false,"given":"Dorota","family":"Suszczyk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1532-2662","authenticated-orcid":false,"given":"Anna","family":"Paw\u0142owska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monika","family":"Bilska","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agata","family":"Chudzik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiktoria","family":"Skiba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Paduch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Kotarski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,9,30]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-017-3501-8"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2016.04.002"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers11091250"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijgo.2013.10.001"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004347-200001000-00003"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2015.11.011"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.25126"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-59009-z"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2020.108334"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/94.8.617"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/08820139.2019.1691222"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar1916"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S179189"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar1918"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1358\/dot.2006.42.9.1025692"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.2006.01750.x"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-11-0945"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.3978\/j.issn.2218-6751.2014.07.01"},{"key":"e_1_2_11_19_2","first-page":"198","article-title":"Cytokiny","author":"Go\u0142\u0105b J.","year":"2002","journal-title":"Immunologia. 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