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The redox couples, GSH\/GSSG and NAD<jats:sup>+<\/jats:sup>\/NADH, play an essential role in physiology as internal defenses against excessive ROS generation by facilitating intracellular\/mitochondrial (mt) redox homeostasis. Anoxia alone and anoxia\/reoxygenation (A\/R) are dissimilar pathological processes. In this study, we measured the impact of capsaicin (Cap) on these pathological processes using a primary cultured neonatal rat cardiomyocyte in vitro model. The results showed that overproduction of ROS was tightly associated with disturbed GSH\/GSSG and NAD<jats:sup>+<\/jats:sup>\/NADH suppressed mt complex I and III activities, decreased oxygen consumption rates, and elevated extracellular acidification rates. During anoxia or A\/R period, these indices interact with each other causing the mitochondrial function to worsen. Cap protected cardiomyocytes against the different stages of A\/R injury by rescuing NAD<jats:sup>+<\/jats:sup>\/NADH, GSH\/GSSG, and mt complex I\/III activities and cellular energy metabolism. Importantly, Cap-mediated upregulation of 14-3-3<jats:italic>\u03b7<\/jats:italic>, a protective phosphoserine-binding protein in cardiomyocytes, ameliorated mt function caused by a disruptive redox status and an impaired ETC. In conclusion, redox pair, mt complex I\/III, and metabolic equilibrium were significantly different in anoxia alone and A\/R injury; Cap through upregulating 14-3-3<jats:italic>\u03b7<\/jats:italic> plays a protection against the above injury in cardiomyocyte.<\/jats:p>","DOI":"10.1155\/2020\/1750289","type":"journal-article","created":{"date-parts":[[2020,3,4]],"date-time":"2020-03-04T23:31:51Z","timestamp":1583364711000},"page":"1-16","source":"Crossref","is-referenced-by-count":19,"title":["Capsaicin Alleviates the Deteriorative Mitochondrial Function by Upregulating 14-3-3<i>\u03b7<\/i> in Anoxic or Anoxic\/Reoxygenated Cardiomyocytes"],"prefix":"10.1155","volume":"2020","author":[{"given":"Yang","family":"Qiao","sequence":"first","affiliation":[{"name":"Jiangxi Provincial Institute of Hypertension, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China"},{"name":"Jiangxi Provincial Key Laboratory of Basic Pharmacology, Nanchang University School of 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