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Generally, dioxin exerts its toxicity via aryl hydrocarbon receptor (AhR). Primary biliary cholangitis (PBC) is a chronic autoimmune disease, and its pathogenesis involves the interplay between immune and environmental factors. This study showed the effect of dendritic cells (DCs) activated by TCDD on na\u00efve CD4<jats:sup>+<\/jats:sup> T cell differentiation in patients with PBC. CD14<jats:sup>+<\/jats:sup> mononuclear cells were isolated from peripheral blood mononuclear cells (PBMCs) of patients with PBC and healthy people by magnetic cell separation and introduced into DCs. Two days after stimulation by TCDD, DCs were cocultured with na\u00efve CD4<jats:sup>+<\/jats:sup> T cells in a ratio of 1\u2009:\u20092 for 3 days. Then, differentiation\u2010related factors for na\u00efve CD4<jats:sup>+<\/jats:sup> T cells were detected by real\u2010time fluorescence quantitative polymerase chain reaction, enzyme\u2010linked immunosorbent assay, and flow cytometry. The results showed that TCDD\u2010activated DCs could promote Th1 and Th17 differentiation in patients with PBC. 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