{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T10:40:40Z","timestamp":1780569640514,"version":"3.54.1"},"reference-count":21,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,1,7]],"date-time":"2020-01-07T00:00:00Z","timestamp":1578355200000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Disease"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cardiology Research and Practice"],"published-print":{"date-parts":[[2020,1,7]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. Hypercholesterolemia- (HC-) induced endothelial dysfunction is the first step of atherogenesis, and the peroxisome proliferator-activated receptor <jats:italic>\u03b3<\/jats:italic> (PPAR<jats:italic>\u03b3<\/jats:italic>) has been reported to attenuate atherosclerosis formation; however, the underlying mechanisms are not fully understood. The present study was designed to determine whether myeloperoxidase (MPO) mediates HC-induced endothelial dysfunction and the role of the PPAR<jats:italic>\u03b3<\/jats:italic> agonist pioglitazone (PIO) in attenuating endothelial dysfunction. <jats:italic>Methods<\/jats:italic>. Male Wistar rats were fed with normal or high cholesterol diets for 8 weeks. HC rats were randomized to receive dapsone (DDS, the MPO inhibitor) during the last 6 days or PIO for the remaining 4 weeks. Vascular endothelial function was determined by comparing vasorelaxation to ACh, an endothelium-dependent vasodilator, and SNP, an endothelium-independent vasodilator in vascular rings in vitro. The vascular MPO activity, NO<jats:sub><jats:italic>x<\/jats:italic><\/jats:sub> content, and cGMP level were measured by the MPO activity assay kit, NO assay kit, and cGMP RIA kit. <jats:italic>Results<\/jats:italic>. Compared with rats fed with normal diet, endothelium-dependent vasodilation, NO<jats:sub><jats:italic>x<\/jats:italic><\/jats:sub> content, and cGMP level were decreased, and MPO activity was increased in thoracic aortas of rats fed with HC diet. There was a negative correlation between vascular endothelial function, NO<jats:sub><jats:italic>x<\/jats:italic><\/jats:sub> content or cGMP level, and MPO activity. PIO obviously reduced the MPO activity, increased NO<jats:sub><jats:italic>x<\/jats:italic><\/jats:sub> content and cGMP level, and improved endothelium-dependent vasodilation function in HC rats, which was essentially the same as that seen with DDS. And, there was a negative correlation between vascular endothelial function, NO<jats:sub><jats:italic>x<\/jats:italic><\/jats:sub> content or cGMP level, and MPO activity in the HC group and the PIO intervention group. <jats:italic>Conclusion<\/jats:italic>. MPO might provoke vascular endothelial dysfunction in hypercholesterolemic rats by reducing the NO biological activity and impairing the NO\/cGMP\/cGK signaling pathway. PIO might inhibit vascular MPO activity and increase NO bioavailability with the net result of reversing endothelial dysfunction.<\/jats:p>","DOI":"10.1155\/2020\/1845969","type":"journal-article","created":{"date-parts":[[2020,1,7]],"date-time":"2020-01-07T18:30:39Z","timestamp":1578421839000},"page":"1-9","source":"Crossref","is-referenced-by-count":8,"title":["The Peroxisome Proliferator-Activated Receptor <i>\u03b3<\/i> Agonist Pioglitazone Protects Vascular Endothelial Function in Hypercholesterolemic Rats by Inhibiting Myeloperoxidase"],"prefix":"10.1155","volume":"2020","author":[{"given":"Dapeng","family":"Zhang","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, Capital Medical University, Beijing 100069, China"},{"name":"Heart Center & Beijing Key Laboratory of Hypertension Disease, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yehong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Physiology, Shanxi Medical University, Taiyuan 030001, Shanxi, China"},{"name":"Department of Gastroenterology, Shuozhou People\u2019s Hospital, Shuozhou 036002, Shanxi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yi","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, Capital Medical University, Beijing 100069, China"},{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suli","family":"Zhang","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, Capital Medical University, Beijing 100069, China"},{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6770-6767","authenticated-orcid":true,"given":"Ye","family":"Wu","sequence":"additional","affiliation":[{"name":"Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, Capital Medical University, Beijing 100069, China"},{"name":"Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gheart.2016.10.024"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.81.2.491"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1161\/01.cir.0000097003.49585.5e"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1161\/01.res.87.10.840"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0104788"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2016-12186"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1172\/jci117342"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1126\/science.1106830"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.53.082901.104018"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m311625200"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2019.02.006"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/s0735-1097(03)00333-4"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.2174\/0929867326666190903112146"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-48382-5_20"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1089\/152308603764816541"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m100191200"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m007659200"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1161\/circulationaha.105.590083"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1161\/hq1201.100261"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1007\/s11883-000-0067-3"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2019.117047"}],"container-title":["Cardiology Research and Practice"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/crp\/2020\/1845969.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/crp\/2020\/1845969.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/crp\/2020\/1845969.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,1,7]],"date-time":"2020-01-07T18:30:43Z","timestamp":1578421843000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/crp\/2020\/1845969\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,7]]},"references-count":21,"alternative-id":["1845969","1845969"],"URL":"https:\/\/doi.org\/10.1155\/2020\/1845969","relation":{},"ISSN":["2090-8016","2090-0597"],"issn-type":[{"value":"2090-8016","type":"print"},{"value":"2090-0597","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,7]]}}}