{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:43:08Z","timestamp":1792442588715,"version":"4.5.0"},"reference-count":13,"publisher":"Wiley","license":[{"start":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T00:00:00Z","timestamp":1591920000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005047","name":"Natural Science Foundation of Liaoning Province","doi-asserted-by":"publisher","award":["2015020536"],"award-info":[{"award-number":["2015020536"]}],"id":[{"id":"10.13039\/501100005047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Journal of Endocrinology"],"published-print":{"date-parts":[[2020,6,12]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. The current treatment for postoperative hypoparathyroidism has shortcomings, such as repeated blood monitoring for dosage adjustment, uncertain long-term efficacy, and the high price of recombinant parathyroid hormone therapy. Adipose-derived stem cells can undergo adipogenic and osteogenic differentiation in vitro and are considered a novel source of parathyroid-like cells, but the idea lacks theoretical basis and feasibility. We aimed at establishing a protocol for differentiating adipose-derived stem cells into parathyroid-like cells for treating hypoparathyroidism. <jats:italic>Materials<\/jats:italic>\/<jats:italic>Methods<\/jats:italic>. Adipose-derived stem cells were isolated and purified from the inguinal adipose tissue of Sprague Dawley rats. Adipogenic differentiation and osteogenic differentiation of the cells were identified by oil red O and alizarin red S staining, respectively. The adipose-derived stem cells were stimulated by sonic hedgehog (SHH) and activin A. The differentiation of the adipose-derived stem cells to parathyroid-like cells was confirmed by the detection of parathyroid hormone and the related parathyroid markers. <jats:italic>Results<\/jats:italic>. Adipose-derived stem cells were successfully isolated and purified from the rat adipocytes. The adipogenic and osteogenic differentiation capabilities of the adipose-derived stem cells were determined. SHH and activin A stimulated parathyroid hormone secretion by the adipose-derived stem cells and significantly increased the expression of calcium-sensing receptor (CaSR), parathyroid hormone, and glial cells missing homolog 2 (GCM2) in the cells in a time- and concentration-dependent manner. <jats:italic>Conclusion<\/jats:italic>. We successfully differentiated rat adipose-derived stem cells into parathyroid-like cells, which will pave a new route to curing hypoparathyroidism.<\/jats:p>","DOI":"10.1155\/2020\/1860842","type":"journal-article","created":{"date-parts":[[2020,6,12]],"date-time":"2020-06-12T23:31:22Z","timestamp":1592004682000},"page":"1-6","source":"Crossref","is-referenced-by-count":7,"title":["Differentiation of Rat Adipose-Derived Stem Cells into Parathyroid-Like Cells"],"prefix":"10.1155","volume":"2020","author":[{"given":"Ping","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7537-8257","authenticated-orcid":true,"given":"Hao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6621-4800","authenticated-orcid":true,"given":"Wenwu","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6022-3909","authenticated-orcid":true,"given":"Zhihong","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0205-5454","authenticated-orcid":true,"given":"Yuan","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9519-7891","authenticated-orcid":true,"given":"Changhao","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0088-7217","authenticated-orcid":true,"given":"Qi","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of General Surgery, The First Hospital of China Medical University, Shenyang, Liaoning Province 110001, China"},{"name":"Department of General Surgery, The People\u2019s Hospital of China Medical University, Shenyang, Liaoning Province 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.29251"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21338"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.9384"},{"key":"4","first-page":"827","volume":"273","year":"2016","journal-title":"Head and Neck 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