{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T00:21:28Z","timestamp":1784247688545,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T00:00:00Z","timestamp":1579478400000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010203","name":"Agriculture Research System of China","doi-asserted-by":"publisher","award":["CARS-38"],"award-info":[{"award-number":["CARS-38"]}],"id":[{"id":"10.13039\/501100010203","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31460670"],"award-info":[{"award-number":["31460670"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p><jats:italic>Brucella<\/jats:italic>\u2010caused brucellosis is one of the most widespread worldwide zoonoses. Lipopolysaccharide (LPS) of <jats:italic>Brucella<\/jats:italic>, which functions as pathogen\u2010associated molecular patterns (PAMPs), is an important virulence factor that elicits protective antibodies. <jats:italic>Per<\/jats:italic> of <jats:italic>B<\/jats:italic>. <jats:italic>melitensis<\/jats:italic> is involved in the biosynthesis of the O\u2010side chain of LPS. Autophagy is a crucial element of the innate immune response against intracellular pathogens including <jats:italic>Brucella<\/jats:italic>. In this study, we observed that autophagy was inhibited in RAW264.7 cells infected with <jats:italic>Brucella melitensis<\/jats:italic> \u2206per. And, a high\u2010throughput array\u2010based screen and qRT\u2010PCR validation were performed to identify the differentially expressed miRNAs in RAW264.7 cells infected with <jats:italic>B. melitensis<\/jats:italic> M5\u201090 \u2206per. The results suggested that <jats:italic>mmu-miR-146a-5p<\/jats:italic>, <jats:italic>mmu-miR-155-5p<\/jats:italic>, <jats:italic>mmu-miR-146b-5p<\/jats:italic>, and <jats:italic>mmu-miR-3473a<\/jats:italic> were upregulated and <jats:italic>mmu-miR-30c-5p<\/jats:italic> was downregulated. During <jats:italic>B. melitensis<\/jats:italic> M5\u201090 \u2206per infection, the increased expression of <jats:italic>miR-146b-5p<\/jats:italic> inhibited the autophagy activation in RAW264.7 cells. Using a bioinformatics approach, <jats:italic>Tbc1d14<\/jats:italic> was predicted to be a potential target of <jats:italic>miR-146b-5p<\/jats:italic>. The results of a luciferase reporter assay indicated that <jats:italic>miR-146b-5p<\/jats:italic> directly targeted the 3\u2032\u2010UTR of <jats:italic>Tbc1d14<\/jats:italic>, and the interaction between <jats:italic>miR-146b-5p<\/jats:italic> and the 3\u2032\u2010UTR of <jats:italic>Tbc1d14<\/jats:italic> was sequence\u2010specific. High\u2010throughput RNA\u2010Seq\u2010based screening was performed to identify differentially expressed genes in Tbc1d14\u2010expressing RAW264.7 cells, and these were validated by qRT\u2010PCR. Among the differentially expressed genes, four autophagy associated genes, <jats:italic>IFN\u03b3-inducible p47 GTPase<\/jats:italic> 1 (<jats:italic>IIGP1<\/jats:italic>), <jats:italic>nuclear receptor binding protein 2<\/jats:italic> (<jats:italic>Nrbp2<\/jats:italic>), <jats:italic>transformation related protein 53 inducible nuclear protein 1<\/jats:italic> (<jats:italic>Trp53inp1<\/jats:italic>), and <jats:italic>immunity-related GTPase family M member 1<\/jats:italic> (<jats:italic>Irgm1<\/jats:italic>), were obtained. Our findings provide important insights into the functional mechanism of LPS of <jats:italic>B. melitensis<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2020\/1953242","type":"journal-article","created":{"date-parts":[[2020,1,20]],"date-time":"2020-01-20T23:31:26Z","timestamp":1579563086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["miR\u2010146b\u20105p Plays a Critical Role in the Regulation of Autophagy in \u2206per <i>Brucella melitensis<\/i>\u2010Infected RAW264.7 Cells"],"prefix":"10.1155","volume":"2020","author":[{"given":"Jiao","family":"Hanwei","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Nie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huapei","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baobao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4411-2293","authenticated-orcid":false,"given":"Feng","family":"Pang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8948-5187","authenticated-orcid":false,"given":"Xiaohong","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiyong","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojian","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongmei","family":"Peng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaying","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohua","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenxing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haifeng","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1711-7793","authenticated-orcid":false,"given":"Kailian","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianjing","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4861-5429","authenticated-orcid":false,"given":"Ying","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5181-6518","authenticated-orcid":false,"given":"Chuangfu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5807-3585","authenticated-orcid":false,"given":"Li","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2043-2703","authenticated-orcid":false,"given":"Fengyang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,1,20]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1056\/nejmra050570"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0302.970219"},{"key":"e_1_2_9_3_2","volume-title":"Veterinary Microbiology","author":"Hirsh D. 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