{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T18:03:34Z","timestamp":1772129014838,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,14]],"date-time":"2020-12-14T00:00:00Z","timestamp":1607904000000},"content-version":"vor","delay-in-days":348,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Technology Development Foundation, Suzhou, China","award":["SYSD2018201"],"award-info":[{"award-number":["SYSD2018201"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Anzi Heji (AZHJ) has been used to treat anticardiolipin antibody\u2010 (ACA\u2010) positive pregnant women at risk of spontaneous abortion for many years. The aim of this study was to investigate the protective mechanism of AZHJ in a mouse model of ACA\u2010positive pregnancy at risk of spontaneous abortion using label\u2010free quantitative proteomics. Mice were divided into three groups: normal pregnant mice (control group), ACA\u2010positive pregnant mice administered normal saline (model group), and ACA\u2010positive pregnant mice administered AZHJ (AZHJ group). The model was established by injecting <jats:italic>\u03b2<\/jats:italic>2\u2010glycoprotein I (GPI) into mice for 18 days. The DEPs and their functions were analyzed by label\u2010free quantitative proteomic and bioinformatic analyses. The levels of IL\u20106, IL\u201010, ACA, and TNF\u2010<jats:italic>\u03b1<\/jats:italic> in the serum and placentas of the mice were measured by enzyme\u2010linked immunosorbent assays (ELISAs). Proteomic data were validated by western blot analysis. The abnormal serum and placental levels of IL\u20106, ACA, and TNF\u2010<jats:italic>\u03b1<\/jats:italic> in the model group were reversed by AZHJ. There were 39 upregulated and 10 downregulated DEPs in the AZHJ group relative to the model group. Bioinformatic analysis revealed that the DEPs were mainly involved in nucleic acid binding, signal conduction, and posttranslational modification. The placental levels of T\u2010cell immunoglobulin mucin 3 (Tim\u20103) and Toll\u2010like receptor 4 (TLR4) expression and AKT phosphorylation in the three groups were consistent with the proteomic findings. Tim\u20103\/AKT signaling is involved in maternal\u2010fetal immune tolerance, while TLR4 is associated with inflammatory responses. Collectively, these results indicate that AZHJ may exert its protective effect in ACA\u2010positive pregnant mice by regulating the maternal\u2010fetal immune tolerance and inflammatory response.<\/jats:p>","DOI":"10.1155\/2020\/1967698","type":"journal-article","created":{"date-parts":[[2020,12,15]],"date-time":"2020-12-15T02:50:09Z","timestamp":1608000609000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Proteomic Analysis of Differentially Expressed Proteins in the Placenta of Anticardiolipin Antibody\u2010 (ACA\u2010) Positive Pregnant Mice after Anzi Heji 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