{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:49:59Z","timestamp":1772164199216,"version":"3.50.1"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T00:00:00Z","timestamp":1596585600000},"content-version":"vor","delay-in-days":217,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002855","name":"Ministry of Science and Technology of the People's Republic of China","doi-asserted-by":"publisher","award":["2018ZX09721003"],"award-info":[{"award-number":["2018ZX09721003"]}],"id":[{"id":"10.13039\/501100002855","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>The present study aims to investigate the effects and mechanisms of sarsasapogenin resistance to precocious puberty. Female Sprague Dawley rats were divided into a normal (N) group, model (M) group, leuprolide (L) group, and sarsasapogenin (Sar) group. Rats at 5 days of age were given a single subcutaneous injection of 300 micrograms of danazol to establish the precocious puberty model. After 10 days of modeling, drug intervention was started. The development of the uterus and ovary was observed by hematoxylin and eosin (HE) staining. The levels of the serum luteinizing hormone (LH), follicle\u2010stimulating hormone (FSH), and estradiol (E2) were determined by radioimmunoassay. Also, the expressions of the hypothalamic gonadotropin releasing hormone (GnRH), Kiss\u20101, G protein\u2010coupled receptor 54 (GPR54), and pituitary gonadotropin releasing hormone receptor (GnRH\u2010R) were detected by RT\u2010PCR. The results showed that compared with the model group, sarsasapogenin could significantly delay the opening time of vaginal, decreased uterine and ovarian coefficients, and reduced uterine wall thickness. Moreover, it can significantly downregulate the levels of serum hormones and reduce the expression of GnRH, GnRH\u2010R, and kiss\u20101. In summary, our results indicate that sarsasapogenin can regulate the HPG axis through the kiss\u20101\/GPR54 system for therapeutic precocious puberty.<\/jats:p>","DOI":"10.1155\/2020\/1978043","type":"journal-article","created":{"date-parts":[[2020,8,5]],"date-time":"2020-08-05T19:33:17Z","timestamp":1596655997000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Study on the Mechanism of Sarsasapogenin in Treating Precocious Puberty by Regulating the HPG Axis"],"prefix":"10.1155","volume":"2020","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7774-1240","authenticated-orcid":false,"given":"Kaili","family":"Hu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenyan","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunyan","family":"Guo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1699-5551","authenticated-orcid":false,"given":"HongSheng","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2136-7410","authenticated-orcid":false,"given":"Xiaoling","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,5]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1136\/adc.70.2.116"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2019.01.003"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1159\/000336325"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1530\/eje-15-0590"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2014.12.021"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3841"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jadohealth.2006.10.002"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-1025"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40272-015-0130-8"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1515\/jpem-2014-0222"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0015-0282(16)46869-x"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.4103\/0366-6999.156773"},{"key":"e_1_2_9_13_2","doi-asserted-by":"publisher","DOI":"10.1080\/17446651.2019.1575726"},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.05.006"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctim.2014.01.002"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2014.02.013"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11011-016-9877-z"},{"key":"e_1_2_9_18_2","first-page":"120","article-title":"Analysis on medication rule of Chinese medicine in treating precocious puberty based on data mining technology","volume":"16","author":"LIu Z.","year":"2019","journal-title":"China Medical Herald"},{"key":"e_1_2_9_19_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/9389028"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellbi.2007.02.001"},{"key":"e_1_2_9_21_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.7254"},{"key":"e_1_2_9_22_2","doi-asserted-by":"publisher","DOI":"10.5555\/afhs.2006.6.2.81"},{"key":"e_1_2_9_23_2","volume-title":"Experimental Study on the Inhibition on the Hpg Axis of Pubertal Rats by Arnebia","author":"Yan Z.","year":"2007"},{"key":"e_1_2_9_24_2","volume-title":"The Effect of Lithospermum Extracts Treatment on Experimental True Precocious Puberty","author":"Song L.","year":"2006"},{"key":"e_1_2_9_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3940(93)90636-y"},{"key":"e_1_2_9_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0015-0282(16)54294-0"},{"key":"e_1_2_9_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13633-016-0022-x"},{"key":"e_1_2_9_28_2","first-page":"367","article-title":"Central precocious puberty: from physiopathological mechanisms to treatment","volume":"28","author":"Chirico V.","year":"2014","journal-title":"Journal of Biological Regulators & Homeostatic Agents"},{"key":"e_1_2_9_29_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2010-2745"},{"key":"e_1_2_9_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2018.05.008"},{"key":"e_1_2_9_31_2","first-page":"21","article-title":"Usefulnes of imaging techniques in the diagnostics of precocious puberty in boys","volume":"76","author":"Jakubowska A.","year":"2011","journal-title":"Polish Journal of Radiology"},{"key":"e_1_2_9_32_2","first-page":"19","article-title":"Precocious puberty: an experience from a major teaching hospital in Central Saudi Arabia","volume":"17","author":"Osman H. A.","year":"2017","journal-title":"Sudanese Journal of Paediatrics"},{"key":"e_1_2_9_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12519-013-0411-7"},{"key":"e_1_2_9_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40618-017-0627-9"},{"key":"e_1_2_9_35_2","doi-asserted-by":"publisher","DOI":"10.1507\/endocrj1954.14.209"},{"key":"e_1_2_9_36_2","doi-asserted-by":"publisher","DOI":"10.1139\/y77-149"},{"key":"e_1_2_9_37_2","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-3-38"},{"key":"e_1_2_9_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2017.03.022"},{"key":"e_1_2_9_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2006.04.015"},{"key":"e_1_2_9_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2012.12.007"},{"key":"e_1_2_9_41_2","doi-asserted-by":"publisher","DOI":"10.1210\/er.2018-00087"},{"key":"e_1_2_9_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0463.2001.tb05758.x"},{"key":"e_1_2_9_43_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0205810"},{"key":"e_1_2_9_44_2","doi-asserted-by":"publisher","DOI":"10.1097\/md.0000000000010267"},{"key":"e_1_2_9_45_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-3031"},{"key":"e_1_2_9_46_2","doi-asserted-by":"publisher","DOI":"10.6065\/apem.2019.24.3.164"},{"key":"e_1_2_9_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yfrne.2015.04.002"},{"key":"e_1_2_9_48_2","doi-asserted-by":"publisher","DOI":"10.1177\/0009922814541807"},{"key":"e_1_2_9_49_2","doi-asserted-by":"publisher","DOI":"10.1159\/000124831"},{"key":"e_1_2_9_50_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci116142"},{"key":"e_1_2_9_51_2","doi-asserted-by":"publisher","DOI":"10.1517\/13543784.16.11.1851"},{"key":"e_1_2_9_52_2","doi-asserted-by":"publisher","DOI":"10.1097\/md.0000000000000260"},{"key":"e_1_2_9_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2017.10.015"},{"key":"e_1_2_9_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/88.23.1731"},{"key":"e_1_2_9_55_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11154-006-9020-2"},{"key":"e_1_2_9_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2016.08.012"},{"key":"e_1_2_9_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409330102"},{"key":"e_1_2_9_58_2","doi-asserted-by":"publisher","DOI":"10.1159\/000493406"},{"key":"e_1_2_9_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0014-5793(99)00009-5"},{"key":"e_1_2_9_60_2","doi-asserted-by":"publisher","DOI":"10.1159\/000083140"},{"key":"e_1_2_9_61_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.m805251200"},{"key":"e_1_2_9_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2016.04.020"},{"key":"e_1_2_9_63_2","doi-asserted-by":"publisher","DOI":"10.1038\/aps.2010.85"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2020\/1978043.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2020\/1978043.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2020\/1978043","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,7]],"date-time":"2024-08-07T04:44:48Z","timestamp":1723005888000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2020\/1978043"}},"subtitle":[],"editor":[{"given":"Tadaaki","family":"Satou","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,1]]},"references-count":63,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.1155\/2020\/1978043"],"URL":"https:\/\/doi.org\/10.1155\/2020\/1978043","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.21203\/rs.3.rs-16977\/v1","asserted-by":"object"}]},"ISSN":["1741-427X","1741-4288"],"issn-type":[{"value":"1741-427X","type":"print"},{"value":"1741-4288","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1]]},"assertion":[{"value":"2020-04-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-07-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"1978043"}}